基于磁性纳米材料构建酶联增敏生物传感器检测双酚A  

Enzyme-linked sensitization biosensor detection of bisphenol A in food matrix Based on magnetic nanomaterials

作　　者：许宙[1] 唐瑶[1] 汪荣 陈茂龙[1] 文李[1] 程云辉[1] 

机构地区：[1]长沙理工大学化学与生物工程学院,湖南长沙410114

出　　处：《食品与机械》2017年第10期47-51,共5页Food and Machinery

基　　金：国家自然科学基金(编号:31401566;31601550);国家重点研发计划(编号:2016YFF0203701)

摘　　要：以纳米材料为载体,以双酚A适配体及互补链为生物识别单元,建立了基于功能化磁纳米粒子及金纳米粒子的辣根过氧化物酶酶联增敏检测平台,可实现对食品基质中双酚A的快速前处理和痕量检测,该方法将可与双酚A特异性结合的适配体偶联于磁性纳米材料上,再与适配体互补链及辣根过氧化物酶(HRP)修饰的金纳米粒子进行竞争性结合,通过HRP对底物的催化水解反应引起450nm处特征峰的变化来定量检测双酚A。结果表明:该检测体系在0~100ng/mL时具有线性关系(R^2=0.978 4),检测限低至0.5pg/mL,具有较好的实用性,为更好地检测食品基质中的双酚A提供了有力的技术支持。A horseradish peroxidase enzyme-sensitized detection plat-form based on functionalized magnetic nanoparticles and gold nanop-articles was established with the nanomaterials as the carrier and the bisphenol A aptamer and the complementary strand as the biometric recognition unit. The method about rapid pretreatment and trace detection of bisphenol A in food matrix was established. The BPA aptamer binding to the magnetic nanomaterials could compete with the complementary strands of the aptamer and the horn root peroxi- dase （HRP） modified gold nanoparticles. The effect of HRP on the catalytic hydrolysis of the substrate resulted in the change of charac-teristic peaks at 450 nm, helping to quantitatively detect bispheool A, which had the advantages of good selectivity, high sensitivity and good specificity. The results showed that this detection system had a linear relationship （R2 = 0.978 4） in the concentration range of 0-100 ng/mL, and the limit of detection was as low as 0.5 pg/mL. This work provideed strong technical support for detection of bisphenol A in future.

关 键 词：纳米材料 适配体 辣根过氧化物酶 双酚A 

分 类 号：TB383.1[一般工业技术—材料科学与工程] TP212.3[自动化与计算机技术—检测技术与自动化装置]