三聚氰胺-铜(Ⅱ)配合物抑制AT-双链铜纳米颗粒合成荧光检测三聚氰胺（英文）  

Fluorescent Sensor for Melamine Based on Its Copper Complex Interrupted of AT-dsDNA Copper Nanoparticles

作　　者：欧丽娟 孙爱明 吕小龙 陈思羽 王凌云 罗建新 

机构地区：[1]湖南工学院材料与化学工程学院,绿色建筑功能材料实验室,湖南衡阳421002

出　　处：《光谱学与光谱分析》2017年第12期3947-3952,共6页Spectroscopy and Spectral Analysis

基　　金：the NSF of China(21205035);Undergraduate Innovative Test Program of Hunan Institute of Technology(HX1630)

摘　　要：基于三聚氰胺与铜离子配位反应并抑制AT-双链铜纳米颗粒合成,构建了一种新型的"turn-off"策略检测三聚氰胺。当三聚氰胺存在时,与铜离子发生配位反应,使得后期合成铜纳米颗粒的铜离子浓度不够,导致铜纳米颗粒荧光减弱。在最优化实验条件下,对三聚氰胺检测的线性范围为1~150μmol·L^(-1),检出限达0.5μmol·L^(-1)。此外,该方法还可以检测牛奶样品中的三聚氰胺,回收率良好。A novel turn-off strategy was developed for the melamine （Mel） detection based on melamine-Cu conjugate and subsequent interrupted the formation of AT-dsDNA copper nanoparticles （AT-dsDNA CuNPs） . The coordination of melamine to copper cannot make the reduction of Cu2＋ to Cu0 enough in the AT24-CuNPs synthesis process ,and thereby results in the fluorescence intensity of CuNPs decreasing .Under the optimum conditions ,the concentration of melamine showed a good linear relation with the fluorescence response of CuNPs in the range of 1~150μmol ＆#183; L -1 .The detection limit was 0.5μmol ＆#183; L -1 .Furthermore ,good recov-eries were obtained while the proposed method was applied to the analysis of melamine in milk samples .

关 键 词：铜纳米颗粒 铜-三聚氰胺配合物 非标记传感器 三聚氰胺检测 

分 类 号：O433.5[机械工程—光学工程]