链格孢菌侵染采后甜瓜果实组织几丁质酶和β-1,3-葡聚糖酶基因表达分析  

Analysis of Expression Profiles of Chitinase and β-1,3-Glucanase Genes in Muskmelon Fruit Tissue Inoculated with Alternaria alternata

作　　者：白羽嘉 张培岭 黄伟 冯作山 李梦 BAI Yujia;ZHANG Peiling;HUANG Wei;FENG Zuoshan;LI Meng(College of Food Science and Pharmacy, Xinjiang Agricultural University, ürümqi 830052, Chin)

机构地区：[1]新疆农业大学食品科学与药学学院,新疆乌鲁木齐830052

出　　处：《食品科学》2018年第2期185-191,共7页Food Science

基　　金：新疆农业大学校前期资助课题(XJAU201421)

摘　　要：以伽师瓜(抗病性强)和86-1甜瓜(抗病性一般)果实为实验材料,研究链格孢菌(Alternaria alternata)侵染后几丁质酶(chitinase,CHT)和β-1,3-葡聚糖酶(β-1,3-glucanase,GLU)基因表达规律。甜瓜果实接种A.alternata,7℃贮藏,测定病斑大小,荧光定量聚合酶链式反应测定CmCht1、CmCht2和CmGlu相对表达量。结果表明:伽师瓜和86-1甜瓜接种A.alternata,在侵染9 d时出现病斑,9~24 d病斑直径不断扩大,86-1甜瓜病斑直径大于伽师瓜,侵染24 d时86-1甜瓜病斑直径是伽师瓜的1.12倍,伽师瓜对A.alternata侵染的抵抗能力要强于86-1甜瓜。在侵染期间,伽师瓜和86-1甜瓜CmCht1、CmCht2和CmGlu相对表达量显著提高,呈先升高后降低的变化趋势,伽师瓜和86-1甜瓜CmCht1、CmCht2和CmGlu相对表达量最高峰出现时间分别相差3、6、3 d,伽师瓜CmCht1、CmCht2相对表达量始终高于86-1甜瓜,CmGlu相对表达量在侵染12~24 d高于86-1甜瓜。在侵染期间CmCht1、CmCht2和CmGlu表达规律存在差异,侵染前期CmCht1、CmCht2相对表达量较高,侵染中期和后期CmGlu相对表达量较高;A.alternata侵染前期主要诱导了CHT基因的表达,中期和后期主要诱导了GLU的基因表达,CHT和GLU的基因协同表达,起到抗病性的作用。伽师瓜抗病性强于86-1甜瓜与CHT和GLU的基因的表达密切相关。Two different disease resistance muskmelon cultivars（Jiashi and 86-1） were analyzed for the expression profiles of chitinase（CHT） and β-1,3-glucanase（GLU） genes in fruit tissues inoculated with Alternaria alternata.Lesion size was measured and the expression levels of CmCht1,CmCht2 and CmGlu genes were investigated by fluorescent quantitative PCR after subsequent storage at 7 ℃.The results revealed that lesion appeared on the 9 th d after infection and expanded until the 24 th day.Lesion size in 86-1 muskmelon was 1.12 times greater than in Jiashi muskmelon on the 24 th day,illustrating that the latter was more resistant to infection with A.alternate than the former.During the storage period,the relative expression levels of CmCht1,CmCht2,and CmGlu genes were significantly increased,initially rising and then declining.The peak expression levels of CmCht1,CmCht2,and CmGlu genes in the cultivars appeared at intervals of 3,6 and 3 days,respectively.Moreover,the relative expression levels of CmCht1 and CmCht2 were higher in Jiashi muskmelon than in 86-1 muskmelon during the whole storage period,while the relative expression level of CmGlu was higher in Jiashi muskmelon than in 86-1 muskmelon during 12–24 days of storage.The expression profiles of these three genes were different during the storage period.The relative expression levels of CmCht1 and CmCht2 were high during the early period suggesting that A.alternata induced CHT gene expression,whereas that of CmGlu gene was high during the middle and late period suggesting that A.alternata induced GLU gene expression.Disease resistance in muskmelon was due to the coordination of CHT and GLU expression.Therefore,Jiashi muskmelon revealed stronger disease resistance than 86-1 muskmelon,which was closely related to the expression of CHT and GLU.

关 键 词：甜瓜 链格孢菌 几丁质酶 β-1 3-葡聚糖酶 基因表达 

分 类 号：S436.5[农业科学—农业昆虫与害虫防治]