EB病毒抗体、巨细胞病毒抗体与复发缓解型多发性硬化的关系研究  

Study of the correlation of EB virus antibody,cytomegalovirus antibody and relapsing-remitting multiple sclerosis

作　　者：刘诗英 谢旭芳[2] 屈新辉[2] 柴文[2] 胡凡[2] 聂凤[2] 吴晓牧 

机构地区：[1]南昌大学研究生院,南昌330006 [2]江西省人民医院神经内科,南昌330006

出　　处：《江西医药》2017年第12期1261-1264,共4页Jiangxi Medical Journal

基　　金：2012年度国家临床重点专科建设项目(No.卫办医政函[2012]649号);2013年省优势科技创新团队建设计划项目(20133BCB24017);2014年江西省卫生厅科技计划项目(20143007)

摘　　要：目的探讨EB病毒(Epstein-Barr virus,EBV)抗体、巨细胞病毒(Cytomegalovirus,CMV)抗体与复发缓解型多发性硬化(relapsing-remitting multiple sclerosis,RRMS)的关系。方法 26例RRMS患者(RRMS组)和30例健康体检人群(对照组)应用间接酶联免疫吸附法(enzyme-linked immunosorbent assay,ELISA法)检测血清EBV的早期抗原(VEA)-IgG、VEA-IgA、VEA-Ig M和衣壳抗原(VCA)-IgG、VCA-IgA、VCA-Ig M,以及CMV-IgG、CMV-Ig M。检测的6个EBV抗体出现阳性的样本即为EBV抗体阳性样本,EBV抗体阳性率为EBV抗体阳性样本与总样本数比值×100%。对比RRMS组和对照组间各抗体阳性率的差别,以及RRMS组和对照组中CMV-IgG阳性与CMV-IgG阴性之间EBV各抗体阳性率的差别。结果与对照组比较,RRMS组的VCA-IgG、EBV抗体和CMV-IgG阳性率明显增高(P<0.05)。在RRMS组中,CMV-IgG阳性患者的VCA-IgG阳性率比CMV-IgG阴性患者明显降低(55.6%比100%,P<0.05),而在对照组中,CMV-IgG阳性者EBV抗体的阳性率比CMV-IgG阴性患者更高(75.0%比33.3%,P<0.05)。相关性分析:VCA-IgG阳性和EBV抗体阳性与RRMS患病呈正相关(分别r=0.292,P<0.05和r=0.277,P<0.05),VCA-IgG阳性与RRMS患者EDSS呈负相关(r=-0.470,P<0.05),EBV抗体阳性与与RRMS患者EDSS呈正相关(r=0.490,P<0.05)。结论 EBV感染与RRMS的发病有关,但与RRMS复发次数无关。在RRMS中,CMV-IgG阴性患者比CMV-IgG阳性患者更易出现VCA-IgG阳性(即既往EBV感染率更高);而对照组中则相反,CMVIgG阳性的健康体检者EBV感染率(EBV抗体阳性率)更高,提示CMV-IgG有可能减少RRMS患者的EBV感染机会或者其EBV抗体的产生,从而减轻其临床症状。Objective To investigate the correlation of the EB virus(Epstein-Barr virus,EBV),cytomegalovirus(Cytomegalovirus,CMV) antibody and relapsing-remitting multiple sclerosis(relapsing-remitting multiple,sclerosis,RRMS). Methods Serum specimens were collected from the 26 patients with RRMS(RRMSgroup) and 30 health examination cases(controls group).EBV early antigen(VEA)-IgG,VEA-IgA,VEA-Ig M and capsid antigen(VCA)-IgG,VCA-IgA,VCA-Ig M,CMV-IgG and CMVIg M were detected by indirect Enzyme-linked Immunosorbent Assay(ELISA). The positive samples of 6 EBV antibodies tested were defined as positive samples of EBV antibody. The positive rate of EBV antibody was the ratio positive samples of EBV antibody to total samples ×100%. The difference of the positive rate of each antibody was compared between the two groups. The difference of the positive rates of EBV antibodies was compared between CMV-IgG positive and negative in two groups. Results Compared with the control group,the positive rates of VCA-IgG,EBV antibodies and CMV-IgG in RRMS group was significantly higher(P<0.05). In the RRMS group,the positive rate of VCA-IgG in CMV-IgG positive patients was significantly lower than in CMV-IgG negative patients(55.6% vs 100%,P<0.05). meanwhile,in the control group,the positive rate of EBV antibody of CMVIgG positive was significantly higher than CMV-IgG negative patients(75% vs 33.3%,P<0.05). The correlation analysis:VCA IgG positive and EBV antibody positive were positively correlated with the incedence of RRMS(r=0.292,P<0.05 and r=0.277,P<0.05).There was a negative correlation between VCA-IgG and EDSS in RRMS patients(r=-0.470,P<0.05). There was a positive correlation between EBV antibody and EDSS in RRMS patients(r=0.490,P<0.05). Conclusions EBV infection is related to the incidence of RRMS,but it has nothing to do with the frequency of RRMS recurrence. In RRMS patients,CMV-IgG negative patients were more prone to VCA-IgG positive(EBV infection rate is higher previously.) than in CMV-IgG positive patients,while the control g

关 键 词：复发缓解型多发性硬化 EB病毒 巨细胞病毒 抗体 

分 类 号：R744.51[医药卫生—神经病学与精神病学]