口腔鳞状细胞癌组织中Slug基因表达量与淋巴结转移、侵袭分子表达的相关性研究  

The correlation of Slug gene expression with lymph node metastasis and invasion molecule expression in oral squamous cell carcinoma tissue

作　　者：陆山鸣[1,2] 刘敏[1,2] 张栋华[1,2] 

机构地区：[1]南京医科大学口腔疾病研究江苏省重点实验室 [2]南京医科大学附属口腔医院综合科,江苏南京210029

出　　处：《海南医学院学报》2017年第20期2826-2829,2833,共5页Journal of Hainan Medical University

基　　金：江苏高校优势学科建设工程资助项目(2014-37)~~

摘　　要：目的:研究口腔鳞状细胞癌组织中Slug基因表达量与淋巴结转移、侵袭分子表达的相关性。方法:选择在南京医科大学附属口腔医院手术切除的口腔鳞状细胞癌组织,根据淋巴结转移情况分为伴颈淋巴结转移的口腔鳞状细胞癌组织和无淋巴结转移的口腔鳞状细胞癌组织。检测口腔鳞状细胞癌组织中Slug、上皮间质转化分子、侵袭分子的表达量。结果:伴颈淋巴结转移的口腔鳞状细胞癌组织中Slug、N-cadherin、Vimentin、CD147、OPN、GRP78、SDF-1、CXCR4的蛋白表达量显著高于无淋巴结转移的口腔鳞状细胞癌组织,E-cadherin、P120ctn、ZO-1的蛋白表达量显著低于无淋巴结转移的口腔鳞状细胞癌组织;Slug高表达的口腔鳞状细胞癌组织中N-cadherin、Vimentin、CD147、OPN、GRP78、SDF-1、CXCR4的蛋白表达量显著高于Slug低表达的口腔鳞状细胞癌组织,E-cadherin、P120ctn、ZO-1的蛋白表达量显著低于Slug低表达的口腔鳞状细胞癌组织。结论:口腔鳞状细胞癌组织中高表达的Slug能够促进细胞的上皮间质转化及侵袭,进而参与肿瘤细胞的淋巴结转移。Objective：To study the correlation of Slug gene expression with lymph node metastasis and invasion molecule expression in oral squamous cell carcinoma tissue.Methods：Oral squamous cell carcinoma tissue surgical removed in Affiliated Stomatological Hospital of Nanjing Medical University between March 2015 and April 2017 was selected and divided into the oral squamous cell carcinoma tissue with neck lymph node metastasis and the oral squamous cell carcinoma tissues without lymph node metastasis according to the condition of lymph node metastasis.The expression of Slug,epithelial-mesenchymal transition molecules and invasion molecules in the oral squamous cell carcinoma tissue were detected.Results：Slug,N-cadherin,Vimentin,CD147,OPN,GRP78,SDF-1 and CXCR4 protein expression in oral squamous cell carcinoma tissue with neck lymph node metastasis were significantly higher than those in oral squamous cell carcinoma tissue without lymph node metastasis while E-cadherin,P120 ctn and ZO-1 protein expression were significantly lower than those in oral squamous cell carcinoma tissue without lymph node metastasis;N-cadherin,Vimentin,CD147,OPN,GRP78,SDF-1 and CXCR4 protein expression in oral squamous cell carcinoma tissue with high Slug expression were significantly higher than those in oral squamous cell carcinoma tissue with low Slug expression while E-cadherin,P120 ctn and ZO-1 protein expression were significantly lower than those in oral squamous cell carcinoma tissue with low Slug expression.Conclusion：The highly expressed Slug in oral squamous cell carcinoma tissue can promote the epithelial-mesenchymal transition and invasion of the cells to participate in the lymph node metastasis of tumor cells.

关 键 词：口腔鳞状细胞癌 淋巴结转移 上皮间质转化 SLUG 侵袭 

分 类 号：R739.85[医药卫生—肿瘤]