肺癌病灶中eEF1A1表达量对癌细胞负荷程度及增殖活性的影响  

Effects of eEF1A1 expression on cancer cell load and proliferation activity in lung cancer lesions

作　　者：陈双喜 

机构地区：[1]湖北省浠水县人民医院胸外科,湖北浠水438200

出　　处：《海南医学院学报》2017年第23期3286-3289,共4页Journal of Hainan Medical University

基　　金：黄冈市科学技术研究与开发计划项目黄科字[2014]67号

摘　　要：目的:探讨肺癌病灶中eEF1A1表达量对癌细胞负荷程度及增殖活性的影响。方法:200例在本院接受肺癌根治术的非小细胞肺癌患者纳入为肺癌组,同期在本院接受急诊手术治疗的肺大泡患者78例被纳入为肺大泡组。对比两组病灶组织中eEF1A1及增殖基因表达量的差异,检测血清肿瘤标志物、血管新生指标的含量。采用Pearson检验评估非小细胞肺癌患者病灶组织中eEF1A1表达量与肿瘤标志物、血管新生指标、增殖基因的相关关系。结果:肺癌组病灶组织中eEF1A1的表达量高于肺大泡组;肺癌组患者血清中CYFRA21-1、ProGRP、CEA、SCC Ag、HIF-1α、VEGF的含量高于肺大泡组且与eEF1A1的表达量呈正相关;肺癌组患者病灶组织中LRRC3B、TUSC3、VPS33B mRNA的表达量低于肺大泡组且与eEF1A1的表达量呈负相关,MACC1、RACK1 mRNA的表达量高于肺大泡组且与eEF1A1的表达量呈正相关。结论:非小细胞肺癌组织中存在eEF1A1异常高表达,具体表达量可客观反映肿瘤负荷及肿瘤细胞增殖活性。Objective:To investigate the effects of eEF1A1 expression on cancer cell load and proliferation activity in lung cancer lesions.Methods:200 patients with non-small cell lung cancer who received radical operation for lung cancer in our hospital were enrolled in lung cancer group,and 78 patients with pulmonary bullae who accepted emergency surgical treatment in our hospital during the same period were enrolled in pulmonary bullae group.Differences in eEF1A1 and proliferation gene expression in lesion tissue were compared between the two groups,and the contents of serum tumor markers and angiogenesis indexes were detected.Pearson test was used to evaluate the correlation of eEF1A1 expression with tumor markers,angiogenesis indexes and proliferation genes in the lesions of patients with non-small cell lung cancer.Results:eEF1A1 expression in lesion tissue of lung cancer group was higher than that of pulmonary bullae group;serum CYFRA21-1,ProGRP,CEA,SCC Ag,HIF-1αand VEGF levels of lung cancer group were higher than those of pulmonary bullae group and positively correlated with the eEF1A1 expression;LRRC3B,TUSC3 and VPS33 BmRNA expression in lesion tissue of lung cancer group were lower than those of pulmonary bullae group and negatively correlated with the eEF1A1 expression whereas MACC1 and RACK1 mRNA expression were higher than those of pulmonary bullae group and positively correlated with the eEF1A1 expression.Conclusion:eEF1A1 is highly expressed in non-small cell lung cancer tissues,and the specific expression can objectively reflect the tumor load and the proliferation activity of tumor cells.

关 键 词：肺癌 eEF1A1 肿瘤标志物 血管新生指标 增殖基因 

分 类 号：R734.2[医药卫生—肿瘤]