乳腺癌组织中miRNA-214的表达及其与患者临床病理特征和预后的关系  

Expression of mi RNA-214 in breast cancer tissues and the relationship with clinicopathological features and prognosis

作　　者：陈莉林[1] 卢传辉[1] 王淳阅[1] 庄严阵[1] CHEN Li-Lin;LU Chuan-Hui;WANG Chun-Yue;et al(The First Affiliated Hospital of Xiamen University, Xiamen, Fujian 361001, China)

机构地区：[1]厦门大学附属第一医院,福建厦门361001

出　　处：《中国妇幼保健》2017年第24期6287-6289,共3页Maternal and Child Health Care of China

基　　金：2012年福建省卫生厅青年科研课题资助计划(2012-2-77)

摘　　要：目的探讨乳腺癌组织中miRNA-214的表达及其与患者临床病理特征和预后的关系。方法选取2016年1月-12月间该院手术切除的乳腺癌患者的乳腺癌组织及其边缘5 cm以上的癌旁组织各60例为研究对象,应用实时定量q PCR法检测miRNA-214的表达情况,分析乳腺癌组织中miRNA-214的表达与患者临床病理特征和预后的关系。结果乳腺癌组织中miRNA-214含量为(118.85±28.75),显著低于乳腺癌旁组织的含量(408.42±42.33),差异有统计学意义(t=2.974,P<0.05)。将60例乳腺癌组织中miRNA-214的平均含量作为cut-off值,将其分为高表达组34例,低表达组26例。乳腺癌组织中miRNA-214表达与肿瘤分级、淋巴结转移、Ki-67的表达有关(P<0.05),与年龄、肿瘤大小、是否绝经、雌激素受体(ER)、孕激素受体(PR)、人表皮生长因子受体-2(Her-2)的表达无关(P>0.05)。结论乳腺癌组织中存在miRNA-214的低表达,其表达水平与乳腺癌分级、淋巴结转移和Ki-67的表达有关,通过检测乳腺癌组织中miRNA-214的表达可以反映患者的预后情况。Objective To explore the expression of miRNA-214 in breast cancer tissues and the relationship with clinicopathological features and prognosis.Methods Sixty breast cancer patients underwent surgical resection in the hospital from January to December in 2016,then 60 samples of breast cancer tissue and 60 samples of para-carcinoma tissue of breast cancer lesion （the distance was more than 5 cm） were obtained.Real-time quantitative PCR was used to detect the expression of miRNA-214 in breast cancer tissues and para-carcinoma tissues.The expression of miRNA-214 in breast cancer tissues and the relationship with clinicopathological features and prognosis were analyzed.Results The content of miRNA-214 in breast cancer tissues was （118.85±28.75）,which was statistically significantly lower than that in para-carcinoma tissues （408.42±42.33） （t =2.974,P〈0.05）.Taking the average content of miRNA-214 in 60 cases of breast cancer tissues as cut-off value,the cases were divided into high expression group （34 cases） and low expression group （26 cases）.The expression of miRNA-214 in breast cancer tissues was correlated with tumor grade,lymph node metastasis,and Ki-67 expression （P〈0.05）.The expression of miRNA-214 in breast cancer tissues was not correlated with age,tumor size,menopause or not,expressions of estrogen receptor （ER）,progesterone receptor （PR）,and human epidermal growth factor receptor-2 （Her-2） （P 〉 0.05）.Conclusion The expression of MiRNA-214 in breast cancer tissues is down-regulated,which is related to tumor size,lymph node metastasis,and Ki-67 expression,detection of miRNA-214 in breast cancer tissues can reflect the prognosis of patients with breast cancer.

关 键 词：乳腺癌 miRNA-214 病理 预后 

分 类 号：R737.9[医药卫生—肿瘤]