Corneal Endothelial Cell Loss in Femtosecond Laser-assisted Descemet＇s Stripping Automated Endothelial Keratoplasty： A 12-month Follow-up Study  

作　　者：Yun Feng Hong-Qiang Qu Jing Ren Philipp Prahs Jing Hong 

机构地区：[1]Department of Ophthalmology, Peking University Third Hospital, Peking University Eye Center, Beijing 100191, China [2]Department of Ophthalmology, University of Regensburg, Regensburg 93053, Germany

出　　处：《Chinese Medical Journal》2017年第24期2927-2932,共6页中华医学杂志（英文版）

基　　金：This study was supported by a grant from the National Natural Science Foundation of China （No. 81700799）.

摘　　要：Background：Descemet＇s stripping automated endothelial keratoplasty （DSAEK） surgery offers a more standardized approach and reliable method to create corneal grafts with an instrument such as a microkeratome.With the development of Descemet＇s membrane endothelial keratoplasty,an excellent clinical outcome is seen in the treatment of corneal endothelial dysfunctions,which indicates that thinner corneal graft results in better clinical outcome.With the recent development of the femtosecond laser,ultrathin corneal graft preparation has become possible.This study aimed to report corneal graft endothelial cell loss （ECL） in a large series of cases undergoing DSAEK with femtosecond laser-assisted corneal graft preparation within a 12-month period.Methods：This study was designed as a prospective,noncomparative,interventional case series.Totally 126 consecutive eyes with endothelial failure of 120 patients,who had corneal endothelial decompensation and underwent femtosecond-assisted DSAEK using the VisuMax femtosecond laser system,were included in the study.Central endothelial cell density （ECD） was recorded postoperatively at 2 weeks （n =126）,1 month （n =126）,3 months （n =110）,6 months （n =101）,and 12 months （n =71） and then compared with the preoperative eye bank measurements.Pre-and postoperative central ECDs were evaluated using Heidelberg retina tomography-Ⅲ confocal microscopy.ECL was calculated for each postoperative time point.Graft thickness was examined using anterior segment-optical coherence tomography.Results：Mean preoperative cell count was 3383 ± 350 cells/mm^2.Mean postoperative cell counts were 2382 ± 707 cells/mm^2,2179 ± 685 cells/mm^2,2074 ± 688 cells/mm^2,1884 ± 662 cells/mm^2,and 1723 ± 624 cells/mm^2 at 2 weeks,1,3,6,and 12 months,respectively;these represented the ECL of 29.7 ± 19.7%,35.4 ± 19.5%,38.6 ± 19.8%,44.3 ± 18.9%,and 48.9 ± 18.4% at the each corresponding time point.The mean corneal graft thickness after surgery was 142 ± 48 μm,118 �Background：Descemet＇s stripping automated endothelial keratoplasty （DSAEK） surgery offers a more standardized approach and reliable method to create corneal grafts with an instrument such as a microkeratome.With the development of Descemet＇s membrane endothelial keratoplasty,an excellent clinical outcome is seen in the treatment of corneal endothelial dysfunctions,which indicates that thinner corneal graft results in better clinical outcome.With the recent development of the femtosecond laser,ultrathin corneal graft preparation has become possible.This study aimed to report corneal graft endothelial cell loss （ECL） in a large series of cases undergoing DSAEK with femtosecond laser-assisted corneal graft preparation within a 12-month period.Methods：This study was designed as a prospective,noncomparative,interventional case series.Totally 126 consecutive eyes with endothelial failure of 120 patients,who had corneal endothelial decompensation and underwent femtosecond-assisted DSAEK using the VisuMax femtosecond laser system,were included in the study.Central endothelial cell density （ECD） was recorded postoperatively at 2 weeks （n =126）,1 month （n =126）,3 months （n =110）,6 months （n =101）,and 12 months （n =71） and then compared with the preoperative eye bank measurements.Pre-and postoperative central ECDs were evaluated using Heidelberg retina tomography-Ⅲ confocal microscopy.ECL was calculated for each postoperative time point.Graft thickness was examined using anterior segment-optical coherence tomography.Results：Mean preoperative cell count was 3383 ± 350 cells/mm^2.Mean postoperative cell counts were 2382 ± 707 cells/mm^2,2179 ± 685 cells/mm^2,2074 ± 688 cells/mm^2,1884 ± 662 cells/mm^2,and 1723 ± 624 cells/mm^2 at 2 weeks,1,3,6,and 12 months,respectively;these represented the ECL of 29.7 ± 19.7%,35.4 ± 19.5%,38.6 ± 19.8%,44.3 ± 18.9%,and 48.9 ± 18.4% at the each corresponding time point.The mean corneal graft thickness after surgery was 142 ± 48 μm,118 �

关 键 词：Corneal Transplantation Descemet＇s Stripping Endothelial Keratoplasty Femtoseconds 

分 类 号：R0[医药卫生]