EDNRA基因rs5335多态性与aSAH后迟发性脑血管痉挛的关联性研究  

Association between Polymorphism of Endothelin Receptor Type A and Delayed Cerebral Vasospasm in Patients with Aneurysmal Subarachnoid Hemorrhage

作　　者：朱峰[1] 张鹏[1] 宋建荣[1] 何蓓[1] 梁媛[1] 

机构地区：[1]宝鸡市中心医院神经外科,陕西宝鸡721008

出　　处：《现代检验医学杂志》2017年第6期56-59,共4页Journal of Modern Laboratory Medicine

摘　　要：目的探讨动脉瘤性蛛网膜下腔出血(aSAH)后迟发性脑血管痉挛(DCVS)与内皮素受体A(EDNRA)基因rs5335多态性的关系。方法选择2015年1月~2017年1月在宝鸡市中心医院神经外科治疗的133例aSAH患者纳入研究,按照是否并发DCVS分为DCVS组78例和对照组55例,采用聚合酶链反应-限制性片段长度多态性法检测EDNRA基因rs5335位点多态性。结果 DCVS组和对照组基因型分布均符合Hardy-Weinberg遗传平衡(χ~2=0.295,P=0.863;χ~2=0.652,P=0.722)。在等位基因模型和显性基因模型下,DCVS组和对照组比较,rs5335位点等位基因、基因型分布频率差异均具有统计学意义(χ~2=4.213,P=0.040;χ~2=4.790,P=0.029),隐性基因模型下两组基因型差异无统计学意义(χ~2=1.299,P=0.254)。多因素Logistic回归法分析结果显示对于aSAH患者,携带等位基因C可降低DCVS发生风险(OR=0.572,95%CI 0.401~0.872,P=0.021)。结论 EDNRA基因rs5335位点多态性与aSAH患者发生DCVS相关。Objective To investigate the genetic association between endothelin receptor type A （EDNRA） gene polymor- phism and delayed cerebral vasospasm （DCVS） in patients with aneurysmal subarachnoid hemorrhage （aSAH）. Methods 133 aSAH patients from January 2015 to January 2017 were recruited to participate in the study. According to whether com- bined with DCVS,they were divided into the DCVS group （78 cases） and the control group （55 cases）. Genotype was deter- mined by polymerase chain reaction- restriction fragment length polymorphism combined with DNA direct sequencing tech- nique for the polymorphism of the EDNRA gene. Results Samples of DCVS group and control group both were consistent with Hardy-Weinberg＇ s law of inheritance （ X^2 = 0.295, P = 0. 863 ; Xz = 0.652, P = 0. 722 ）. There were significant differ- ences of EDNRA gene rs5335 polymorphism between DCVS group and control group, under allele model （X^2= 4. 213, P= 0. 040） and the dominant model （X^2 = 4. 790,P= 0. 029）. However, there was no difference of EDNRA gene polymorphism between DCVS group and control group under recessive model （2 = 1. 299, P=0. 254）. Multivariate Logistic regression a- nalysis showed that allele C was protective factor of DCVS for aSAH patients （OR = 0. 572,95 % CI 0. 401 - 0. 872, P = 0. 021）. Conclusion For aSAH natients,EDNRA gene rs5335 nolvmort）hism may closelv related to DCVS.

关 键 词：内皮素受体A 动脉瘤性蛛网膜下腔出血 迟发性脑血管痉挛 基因多态性 

分 类 号：R743.9[医药卫生—神经病学与精神病学] Q786[医药卫生—临床医学]