热休克蛋白70-hom基因+2437T/C多态性与乳腺癌易感性的相关性研究  

Association between Polymorphism of Heat Shock Protein 70-hom Gene+2437T/C and Susceptibility to Breast Cancer

作　　者：王媛媛[1] 艾克东 寇建锋[1] 

机构地区：[1]延安市人民医院心胸肿瘤外科,陕西延安716000

出　　处：《现代检验医学杂志》2017年第6期125-127,131,共4页Journal of Modern Laboratory Medicine

摘　　要：目的探讨陕西地区汉族人群中热休克蛋白(HSP)70-hom(+2437T/C)多态性与乳腺癌的相关性。方法收集136例乳腺癌患者和150例健康体检人员的临床资料,采用聚合酶链反应-限制性内切酶分析(PCR-RFLP)法检测HSP70-hom基因+2437T/C位点多态性。结果 HSP70-hom基因+2437T/C位点基因型和等位基因分布频率在病例组和对照组差异均具有统计学意义(χ~2=7.282,P=0.026;χ~2=6.586,P=0.010)。按照免疫组织化学分型,三阴性乳腺癌患者与对照组比较基因型分布频率差异具有统计学意义(χ~2=9.047,P=0.003);其他类型患者与对照组比较,基因型分布频率差异无统计学意义(χ~2=2.575,P=0.109)。结论对于陕西地区汉族人,HSP70-hom基因+2437T/C多态性可能与乳腺癌遗传易感性相关,尤其是三阴性乳腺癌。Objective To investigate the genetic association between Polymorphism of heat shock protein（HSP）70-hom gene＋2437T/C and susceptibility to breast cancer in the Han descent population of Shaanxi Province.Methods 136 patients with breast cancer and 150 healthy controls without breast cancer were recruited to participate in the study.Genotype was determined by Polymerase chain reaction-restriction enzyme analysis for the polymorphism of the HSP70-hom gene ＋2437T/C.Results The distribution of HSP70-hom genotypes and allelotypes had significant difference between the patients with breast cancer and healthy controls（χ2=7.282,P=0.026;χ2=6.586,P=0.010）.Stratified by immunohistochemistry, the distribution of HSP70-hom genotypes types had significant difference between the patients with triple negative breast cancer（TNBC）and healthy controls（χ2=9.047,P=0.003）.However,there was no significant difference of the distribu-tion of HSP70-hom genotypes between the patients with non-TNBC and healthy controls（χ2=2.575,P=0.109）.Conclu-sion For Han descent population of Shaanxi Province,polymorphism of the HSP70-hom gene ＋2437T/C may associate with the risk of breast cancer,especially for TNBC.

关 键 词：热休克蛋白70 乳腺癌 单核苷酸多态性 遗传易感性 

分 类 号：R737.9[医药卫生—肿瘤] R730.43[医药卫生—临床医学]