caveolin-1 siRNA对成釉细胞中CD147糖基化及MMP-2表达的影响  

Effects of caveolin-1 siRNA on CD147 glycosylation and MMP-2 expression in ameloblasts

作　　者：史璐[1] 李凌云 李纾[2] 高玉光 李蓓蕾[1] 陈颖 

机构地区：[1]郑州大学第一附属医院∥郑州大学口腔医学院,河南郑州450000 [2]山东大学口腔医学院,山东济南250012 [3]滨州医学院口腔医学院,山东滨州256603

出　　处：《暨南大学学报（自然科学与医学版）》2017年第6期524-530,共7页Journal of Jinan University(Natural Science & Medicine Edition)

基　　金：国家自然科学基金项目(U1204813);河南省教育厅科学技术研究重点项目(12A320045)

摘　　要：目的:研究caveolin-1的表达对小鼠成釉细胞CD147糖基化及MMP-2表达的影响.方法:首先利用细胞免疫荧光技术检测体外培养的小鼠成釉细胞中caveolin-1、CD147和MMP-2蛋白的表达;然后利用siRNA技术封闭小鼠成釉细胞中caveolin-1的表达,实时定量荧光PCR和免疫印迹试验分别检测caveolin-1、CD147和MMP-2在转录水平和蛋白水平的表达情况,并统计学分析.结果:caveolin-1、CD147和MMP-2蛋白表达于体外培养的小鼠成釉细胞中;caveolin-1siRNA转染成釉细胞后caveolin-1mRNA和MMP-2mRNA的表达量明显降低,但CD147mRNA的表达量没有明显变化;caveolin-1、高糖型CD147和MMP-2的蛋白表达量均降低.结论:caveolin-1、CD147和MMP-2表达于小鼠成釉细胞中,提示3者参与成釉细胞生物学过程;在体外培养的成釉细胞中,封闭caveolin-1基因的表达可降低HG-CD147和MMP-2的表达.Aim: To investigate the regulatory effects of caveolin-1 on CD147 glycosylation and MMP-2 expression in cultured mouse ameloblasts. Methods: Expressions of caveolin-1、CD147 and MMP-2 proteins in mouse ameloblasts were detected by immunofluorescence cytochemistry in vitro. After the caveolin-1 gene was silenced by siRNA in ameloblasts,quantitative real-time PCR and Western blotting were used to determine the expressions of caveolin-1、CD147 and MMP-2 at transcriptional and protein levels, respectively. Results: caveolin-1、CD147 and MMP-2 were expressed in cultured mouse ameloblasts. Silence of caveolin-1 gene by siRNA significantly decreased caveolin-1 and MMP-2 mRNAlevels; however,no significant difference in CD147 mRNA level was found when it was compared with the control group. The levels of caveolin-1, highly glycosylated CD147 and MMP-2 proteins were significantly decreased. Conclusion: caveolin-1、CD147 and MMP-2 may play a role in the biological activities of cultured mouse ameloblasts. Using the RNA interference strategy,down-regulation of caveolin-1 in cultured mouse ameloblasts could suppress HG-CD147 and MMP-2 expressions.

关 键 词：成釉细胞 CAVEOLIN-1 CD147 MMP-2 SIRNA 

分 类 号：R78[医药卫生—口腔医学]