作物病原真菌的拮抗菌筛选及其活性物质初步鉴定  

Scanning of Antagonistic Bacteria Against Crop Pathogenic Fungi and Primary Identification of the Active Substances

作　　者：胡洪涛 张亚妮 张舒[2] 李金泉[3] 张佑宏 汪本福 曹春霞 任淑惠 朱志刚 姚经武 龙同 黄大野 杨自文 Hu Hongtao;Zhang Yani;Zhang Shu;Li Jinquan;Zhang Youhong;Wang Benfu;Cao Chunxia;Ren Shuhui;Zhu Zhigang;Yao Jingwu;Long Tong;Huang Daye;Yang Ziwen(Hubei Pesticide Engineering Research Center , Wuhan , Hubei , 430064, China;Ph ytosanitary Soil and Fertilizer Institute, Hubei Academy of Agricultural Sciences , Wuhan , Hubei , 430064, China;Institute of Economic Crops, Hubei Academy of Agricultural Sciences Wuhan , Hubei, 430064, China;Ministry of Agriculture Central China Crop Cultivation Science Observation Experimental Station ,Wuhan, Hubei,430064, China;Hubei Provincial Key Laboratory of Germplasm and Genetic Improvement of Food Crops,Wuhan, Hubei , 430064, China)

机构地区：[1]湖北省生物农药工程研究中心,湖北武汉430064 [2]湖北省农业科学院植保土肥研究所,湖北武汉430064 [3]湖北省农业科学院经济作物研究所,湖北武汉430064 [4]农业部华中地区作物栽培科学观测实验站,湖北武汉430064 [5]粮食作物种质与遗传改良湖北省重点实验室,湖北武汉430064

出　　处：《绿色科技》2017年第23期164-167,169,共5页Journal of Green Science and Technology

基　　金：国家重点研发计划项目(编号:2016YFD0200807);湖北省农业创新团队项目(编号:2016-620-000-001-039);湖北省农业科学院基金(编号:2016NKYJJ31)

摘　　要：从湖北、安徽等地的水稻、小麦、豇豆、西瓜等种植区采集土壤,进行了微生物分离和培养。采用对峙培养法,筛选到20株对11种重要作物病原真菌,如水稻稻瘟菌、小麦赤霉菌、豇豆根腐菌、西瓜枯萎菌等,具有不同拮抗活性的细菌,其中2株具有广谱抗真菌活性。利用高效液相色谱和质谱对其中一株菌株的代谢产物进行了制备和结构初步鉴定,发现其主要活性成分为多烯类物质,分子量为803.67。继续深入研究抗菌物质结构和抑菌机理,将有助于新型生物源杀菌剂的开发。Soils were collected from rice, wheat, cowpea and watermelon fields from Hubei and Anhui, et al. , which were used for isolation and culture of microorganisms. Adopting the confrontation culture method, 20 strains of bacteria were obtained with different antagonistic activities to 11 species of important pathogenic fungi, such as Magnaporthegrisea, Fusarium graminearum, Fusarium solani, Fusarium oxsporum, et al. , two of which had broad- spectrum antagonistic activities. Utilizing HPLC and LC- MASS, the metabolites of one strain were prepared and the structures were primarily identified, which revealed that one major antagonistic component was a type of polyenic compound with a molecular weight of 803.67. Further in-depth studies in its structure and antagonistic mechanism would benefit for development of new types of fungicides.

关 键 词：生防菌 稻瘟菌 赤霉菌 抑菌 活性 高效液相色谱 质谱 

分 类 号：S476[农业科学—农业昆虫与害虫防治]