参与胡杨异形叶发生的发育相关基因表达模式及作用  

Expression Patterns and Functions of Genes Related to the Development of Populus euphratica Oliv.Heteromorphic Leaves Morphogenesis

作　　者：秦少伟[1] 李才林 郭忠忠 包亮红 姜仁军[1,2] 张娜 刘占文[1] 赵利峰 Qin Shaowei;Li Cailin;Guo Zhongzhong;Bao Lianghong;Jiang Renjun;Zhang Na;Liu Zhanwen;Zhao Lifeng(College of Life Sciences, Tarim University, Alar, 843300;Key Laboratory of Protection and Utilization of Biological Resources in Tarim Basin, Tarim University, Alar, 843300)

机构地区：[1]塔里木大学生命科学学院,阿拉尔843300 [2]兵团塔里木盆地生物资源保护利用重点实验室,阿拉尔843300

出　　处：《基因组学与应用生物学》2017年第11期4785-4792,共8页Genomics and Applied Biology

基　　金：国家自然科学基金(No.31660298;No.31260275);塔里木大学校长基金博士项目(TDZKBS201501)共同资助

摘　　要：为了阐明胡杨异形叶发生的分子机制,本研究以胡杨披针形叶和宽卵形叶为材料,通过RNA-seq分析了上述两种叶形的基因表达谱,发现在胡杨异形中发生的4个时期中,共有3 991个基因在两种叶形中差异表达,利用权重基因共表达网络分析(WGCNA)发现一个含有583个基因的模块与叶形指数高度相关(r=0.93,p=0.004)。然后我们用Gene Ontology对这些基因的功能进行了注释和富集,发现其中有40个基因与发育密切相关。这40个基因的表达模式和功能分析表明,这些基因可以通过参与极性建成、细胞增殖、细胞生长、信号传导和代谢等生物学过程在胡杨异形叶发生中起重要作用。因此,我们的研究有助于进一步完善叶形发育的调控机制。To reveal the molecular mechanism of Populus euphratica Oliv. heteromorphic leaf morphogenesis, the lanceolate and broad-oval leaves ofP. euphratica were selected as the research materials in this study, and their gene expression profiles were analyzed by RNA-seq. It was found that a total of 3 991 genes were differentially ex pressed between the lanceolate and broad-ovalleaves in the four periods of P. euphratica heteromorphic leaf morphogenesis. Furthermore, a model including 583 genes was also found to be highly correlated with leaf index （r=0.93, p=0.004）. Then the functions of these genes were annotated and enriched by Gene Ontology, and 40 genes of them were found to be closely related to development. According to the expression profiles and functions of the 40 genes, it was found that these genes could play a key role in P. euphratica heteromorphic leaf morphogenesis via participating in the biological processes including polarity specification, cell proliferation, cell growth, signal transduction, metabolism and so on. Therefore, our research would contribute to further improve the regulation mechanism of leaf shape development.

关 键 词：胡杨 异形叶 发育相关基因 表达模式 作用 

分 类 号：Q943.2[生物学—植物学] S792.11[农业科学—林木遗传育种]