MGBA负载脐带血DC诱导特异性CTL对乳腺癌细胞的杀伤作用  

MGBA carrying umbilical cord blood-derived dendritic cells induces specific cytotoxic T lymphocytes against breast cancer cells

作　　者：胡园园[1] 刘佩佩[1] 姜又红[1] 

机构地区：[1]中国医科大学附属第一医院肿瘤研究所,辽宁沈阳110001

出　　处：《中国肿瘤生物治疗杂志》2017年第12期1381-1385,共5页Chinese Journal of Cancer Biotherapy

基　　金：辽宁省科技厅社会发展攻关计划资助项目(No.2011415052-3)~~

摘　　要：目的:观察乳腺珠蛋白(mammaglobin A,MGBA)负载脐带血来源树突状细胞(dendritic cell,DC)诱导产生的细胞毒性T细胞(cytotoxic T lymphocyte,CTL)对乳腺癌细胞的体外杀伤效果。方法:采集健康剖宫产女性志愿者的脐带血,分离脐带血单个核细胞并诱导DC生成,用MGBA致敏DC与自体淋巴细胞共培养诱导CTL。采用流式细胞术测定DC的表型(CD83、CD86、HLADR)变化,ELISA法测定IL-10、IL-12分泌水平,CCK-8法测定CTL对乳腺癌细胞的杀伤活性。结果:成功培养出形态典型、功能成熟的DC,MGBA负载DC诱导的MGBA特异性CTL对乳腺癌细胞MDA-MB-415产生显著的杀伤效果(P<0.05);加入HLA-I抗体可显著减弱杀伤效果,加入HLA-II抗体细胞毒活性无显著变化,加入HLA-I抗体或HLA-II抗体对正常乳腺细胞的杀伤性均无影响。结论:MGBA能明显加强脐带血DC诱导的CTL对乳腺癌细胞的杀伤活性,该杀伤活性具有MHC限制性。Objective： To investigate the in vitro cytotoxicity of cytotoxic T lymphocytes （CTLs） induced from umbilical cord blood derived dendritic cells （DCs） that sensitized by mammaglobin A （MGBA） （MGBAp-DCs） against breast cancer. Methods： Umbilical cord blood （UCB） samples were obtained from healthy pregnant women during cesarean delivery and used to isolate UCB mononuclear cells to induce DC. MGBAp-DCs were co-cultured with autologous lymphocytes to induce CTL. The DC phenotypes （CD83, CD86, HLA-DR） were determined by using flow cytometry; and IL-10, IL-12 secretion levels was determined by ELISA; the killing effect of CTL against breast cancer cells was detected by CCK-8. Results： Mature DCs were successfully isolated with typical morphology and functionality characteristics. Specific CTL induced by MGBAp- DCs had a significant cytotoxic effect against breast cancer MDA-MB-415 cells （P〈0.05）. Cytotoxic effect was significantly weakened after adding anti-HLA-I antibody, but not with adding anti-HLA-II antibody. However, the the cytotoxicity of specific CTL on normal breast cell line MCF-10 was not influenced by the presence of anti-HLA-I or anti-HLA-II antibodies or not. Conclusion： MGBAp-DCs can effectively enhance the cytotocxity of CTL against breast cancer cells in vitro.

关 键 词：脐带血 树突状细胞 乳腺珠蛋白 乳腺癌细胞 细胞毒性T细胞 

分 类 号：R730.51[医药卫生—肿瘤] R737.9[医药卫生—临床医学]