miR-125b调控ART4蛋白对肝癌细胞增殖和侵袭的影响  

Effects of miR-125b on proliferation and invasion of hepatocarcinoma cells by ART4 protein

作　　者：李建华[1] 韩玲[1] 杨志良[1] LI Jian-Hua;HAN Ling;YANG Zhi-Liang(Department of General Surgery, Xinxiang Central Hospital, Xinxiang 453000,Chin)

机构地区：[1]新乡市中心医院普外科,新乡453000

出　　处：《中国免疫学杂志》2017年第12期1765-1770,共6页Chinese Journal of Immunology

基　　金：河南省医学科技攻关计划基金资助项目(201602362)

摘　　要：目的:探讨miR-125b对ART4蛋白的调控作用及在肝癌细胞中对其增殖和侵袭能力的影响。方法:q PCR检测肝癌组织和肝癌细胞中miR-125b的表达情况;过表达miR-125b后q PCR检测ART4的表达;双荧光素酶实验检测miR-125b和ART4的关系;MTT实验检测过表达miR-125b对肝癌细胞增殖能力的影响;Transwell侵袭实验检测miR-125b的过表达对肝癌细胞侵袭能力的影响;MTT实验检测过表达ART4后逆转miR-125b对肝癌细胞增殖能力的影响;Transwell侵袭实验检测过表达ART4后逆转miR-125b对肝癌细胞侵袭能力的影响。结果:miR-125b在肝癌细胞中表达水平较低,过表达miR-125b可以有效抑制ART4蛋白的表达;过表达miR-125b可以抑制肝癌细胞的增殖侵袭能力;过表达ART4后可以逆转肝癌细胞被miR-125b抑制的增殖侵袭能力。结论:miR-125b在肝癌中表达下调,同时miR-125b可以调控ART4的表达影响肝癌细胞增殖和侵袭能力。Objective：To investigate the effect of miR-125 b on ART4 protein and its effect on proliferation and invasion of hep-atocellular carcinoma cells .Methods：The expression of miR-125 b in hepatocellular carcinoma and hepatocellular cells was detected by qPCR.The expression of miR-125b and ART4 was detected by qPCR after overexpression of miR-125b.The expression of miR-125b and ART4 was detected by double luciferase assay .The effect of miR-125 b on the proliferation of hepatoma cells was detected by MTT assay.The effect of miR-125b on the invasion of hepatoma cells was detected by Transwell invasion assay .MTT assay was used to detect the effect of miR-125 b on the proliferation of hepatoma cells after overexpression of ART 4.Transwell invasion assay was used to detect the effect of miR-125 b on the invasion of hepatoma cells after overexpression of ART 4.Results： The expression of miR-125 b in hepatoma cells was low,and overexpression of miR-125b could inhibit the expression of ART4 protein.Overexpression of miR-125b could inhibit the proliferation and invasion of hepatoma cells .Overexpression of ART4 could reverse the proliferation and invasion of hepatoma cells by miR-125b.Conclusion：Expression of miR-125b in hepatocellular carcinoma was down-regulated.Meanwhile,miR-125 b can regulate the expression of ART 4 and affect the proliferation and invasion of hepatoma cells .

关 键 词：miR-125b 肝癌 ART4 TRANSWELL 

分 类 号：R735.7[医药卫生—肿瘤]