miR-143调控PAN3蛋白的表达对B细胞淋巴瘤细胞侵袭和迁移的影响  

Effects of miR-143 on invasion and migration of B cell lymphoma cells through regulating expression of PAN3 protein

作　　者：杨满[1] 吴隼[1] 张灵秀[1] 黄琰[1] 字友梅[1] 张媛[1] YANG Man;WU Sun;ZHANG Ling-Xiu;HUANG Yan;ZI You-Mei;ZHANG Yuan(Department of Hematology, First Affiliated Hospital of Xinxiang Medical College, Weihui 453100, China)

机构地区：[1]新乡医学院第一附属医院血液科,卫辉453100

出　　处：《中国免疫学杂志》2017年第12期1774-1778,共5页Chinese Journal of Immunology

基　　金：河南省教育厅基金资助项目(No.2006320039)

摘　　要：目的:探讨miR-143调控PAN3蛋白的表达对B细胞淋巴瘤细胞侵袭和迁移的影响。方法:运用q PCR实验检测miR-143在正常骨髓组织和淋巴瘤血液组织中的表达情况;检测在不同种类的细胞株中miR-143的表达水平;q PCR检测miR-143对PAN3的表达水平的影响;双荧光素酶实验检测miR-143和PAN3之间的相互关系;划痕实验和Transwell侵袭实验检测miR-143表达对B细胞淋巴瘤E6-1细胞迁移和侵袭能力的影响。结果:和正常骨髓组织相比,miR-143在B细胞淋巴瘤中表达明显下调;选取miR-143表达水平较低的E6-1细胞株;双荧光素酶实验表明miR-143可以下调PAN3的表达水平,直接影响PAN3的表达活性;过表达miR-143后,E6-1细胞的侵袭和迁移能力明显受到抑制,抑制E6-1细胞的侵袭转移能力。结论:miR-143可以通过调控PAN3的表达,从而调节B细胞淋巴瘤细胞的迁移和侵袭行为。Objective：To investigate the effect of miR-143 on the invasion and migration of B cell lymphoma cells .Methods：The expression of miR-143 in normal bone marrow and lymphoma was detected by qPCR .The expression levels of miR-143 in different cell lines were examined by qPCR .qPCR was used to detect the ability of miR-143 on PAN3.The relationship between miR-143 and PAN3 was detected by double luciferase assay .The effect of miR-143 expression on the migration ability of B cell lymphoma E 6-1 cells was examined by scratch test .The effect of miR-143 expression on the invasion ability of B cell lymphoma E 6-1 cells was exa mined by transwell test.Results：Compared with normal bone marrow ,miR-143 was down-regulated in B-cell lymphoma.Double luciferase assay showed that miR-143 could regulate the expression of PAN 3.Overexpression of miR-143 ,E6-1 cells significantly reduced the ability to attack and migrate.Conclusion：miR-143 can regulate the migration and invasion of B cell lymphoma cells by regulating the expression of PAN3.

关 键 词：MIR-143 淋巴瘤 PAN3 TRANSWELL 

分 类 号：R733.4[医药卫生—肿瘤]