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作 者:庞盼 张峰波[2] 朱玥洁[3] 贾斌[4] 张跃新[4] 张春桃[1] 甫拉提.热西提 丁剑冰[1] PANG Pan;ZHANG Feng-Bo;ZHU Yue-Jie;JIA Bin;ZHANG Yue-Xin;ZHANG Chun-Tao;REXITI · Fulati;DING Jian-Bing(College of Preclinical Medicine ,Xinjiang Medical University, Urumqi 830011, China)
机构地区:[1]新疆医科大学基础医学院,乌鲁木齐830011 [2]新疆医科大学第一附属医院检验科,乌鲁木齐830011 [3]新疆医科大学第一附属医院生殖助孕科,乌鲁木齐830011 [4]新疆医科大学第一附属医院感染科,乌鲁木齐830011
出 处:《中国免疫学杂志》2017年第12期1838-1842,共5页Chinese Journal of Immunology
基 金:国家自然科学基金项目(81560322);新疆维吾尔自治区自然科学基金项目(2015211C100);新疆医科大学科学创新基金(XYDCX201507)
摘 要:目的:研究程序性死亡受体1(PD-1)/与配体(PD-L1)以及相关细胞因子转化生长因子-β(Transforming growth factor-β,TGF-β)和白细胞介素-10(Interleukin-10,IL-10)在布鲁氏菌病(Brucellosis)患者中的变化。方法:选取60例布鲁氏菌病患者与60例健康志愿者,采用流式细胞术分别检测其外周血单个核细胞(PBMC)中CD3+T细胞、CD4+T细胞、CD8+T细胞中PD-1的表达,以及树突状细胞(Dendritic cell,DC)中PD-L1的表达,同时采用流式液相多重蛋白定量技术(Cytometric bead array,CBA)检测血清中TGF-β和IL-10的变化,采用Pearson法分析布鲁氏菌病患者体内PD-1与TGF-β及IL-10的相关性,并对34例布鲁氏菌病治愈患者进行了追踪随访。结果:与正常对照组相比,布鲁氏菌病患者组外周血中PD-1、PD-L1的表达及血清中TGF-β和IL-10的水平均明显升高(P<0.01),而治愈组外周血中PD-1、PD-L1的表达及血清中TGF-β和IL-10的水平较治疗前明显降低(P<0.01),并且布鲁氏菌病患者组PD-1的表达与TGF-β(r=0.817,P<0.01)及IL-10均呈正相关(r=0.835,P<0.01)。结论:PD-1/PD-L1及TGF-β和IL-10在布鲁氏菌病患者体内高表达,可能共同参与了布鲁氏菌对宿主免疫应答的负性调控作用。Objective: To investigate the roles of PD-1 and PD-L1,as well as related cytokines TGF-β and IL-10 in patients infected with Brucella. Methods: The expression of PD-1/PD-L1 in peripheral blood mononuclear cell CD3+T cells,CD4+T cells,CD8+T cells,and dendrites cells was detected by flow cytometry in 60 patients infected with Brucella and 34 patients receiving follow-up cured and 60 healthy controls,the serum levels of TGF-β and IL-10 were detected by CBA. The correlations of PD-1 with TGF-β and IL-10 levels in patients infected with Brucella were analyzed by pearson correlation test. Results: Compared with control,brucellosis patients had the higher PD-1/PD-L1,TGF-β and IL-10 expression( P 〈 0. 05),while in the patients receiving follow-up cured,the PD-1/PDL1,TGF-β and IL-10 were remarkably lower than those in pretherapy( P〈0. 01),the change of PD-1 was positively correlated with the levels of TGF-β( r = 0. 817,P〈0. 01) and IL-10( r = 0. 835,P〈0. 01). Conclusion: High expression of PD-1/PD-L1 and TGF-β and IL-10 in patients with Brucella may be involved in the negative regulation of Brucella on host immune response.
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