miR-143在PTC患者中的表达与对患者免疫功能的影响  

Expression of miR-143 in patients with PTC and its influence on immune function

作　　者：江珊[1] 林梃[1] 陈幸生[1] JIANG Shah;LIN Ting;CHEN Xing-Sheng.(Thyroid Surgery, Fujian Medical University Union Hospital, Fuzhou 350005, Chin)

机构地区：[1]福建医科大学附属协和医院甲状腺外科,福州350005

出　　处：《中国免疫学杂志》2017年第12期1862-1866,共5页Chinese Journal of Immunology

基　　金：福建省科技计划项目(No.2017Y0036)

摘　　要：目的:研究miR143的表达水平与甲状腺乳头状瘤患者(PTC)预后、细胞生物学特性之间的关系,并对相应机制进行探讨。方法:首先使用QPCR检测30例PTC患者癌组织、癌旁组织中miR143的表达水平,并同时调取TCGA数据库中PTC患者生存信息,并检测miR143对生存率与转移率的影响。PTC细胞系IHH-4随后分别转染CON与p Genesil1-miR143载体,48 h后QPCR检测miR143的变化水平,CCK8法检测细胞增殖、Annexin V/PI法检测细胞凋亡、Transwell法检测细胞迁移。随后检测2013年4月至2016年12月之间收治的200名甲状腺乳头状癌患者血清中miR143、IL-10、IFN-γ,并计算皮尔森积矩系数,最后,将患者分为miR143高表达与低表达组,并计算IL-10、IFN-γ的表达量。结果:miR143在癌旁组织与癌组织中的相对表达量分别为(1.0±0.15)vs(0.12±0.06),miR143的表达同时与患者生存率、转移率密切相关(P<0.001)。IHH-4p Genesil1-miR143与CON组的miR143表达水平分别为8.63±0.71 vs 1.0±0.06,在48 h时,p Genesil1-miR143组增殖指数显著低于CON(P<0.01),p Genesil1-miR143组与CON组Annexin V+阳性细胞比例分别为(40±7.2)vs(2±0.38),迁移细胞数的比值分别为(40±7.2)vs(2±0.38),以上P值均小于0.001。在PTC患者中,IL-10、IFN-γ中,r2分别为-0.4,0.62(P<0.001),miR143低表达组与高表达组IFN-γ的表达量分别为(8±0.23)vs(11.2±0.12),IL-10(12±3.1)vs(8.43±0.44)(P<0.05)。结论:miR143在PTC患者中低表达,且与患者预后、生存相关。miR143的高表达使得IHH-4细胞系增殖减弱、凋亡增加、迁移降低,同时miR143的表达与患者的免疫功能相关。Objective：To investigate the relationship between the expression level of miR 143 and prognosis/cellular biological characteristics in patients with papillary thyroid carcinoma （PTC）,and explore the potential mechanisms.Methods：30 PTC patients were admitted and enrolled into the trial from 2013 April to 2016 December.The qPCR was performed to detect the expression of miR143 in cancer tissues and adjacent tissues from 30 PTC patients.The effect of miR143 on overall survival rate and metastasis-free survival rate was assessed with specific analysis .CON and pGenesil1-miR143 vector were transfected into PTC cell line IHH-448h after qPCR.CKK8 and Annexin V/PI flow cytometry were performed to detect cell proliferation ,and apoptosis,respectively.Cell migration was asseessed by transwell experiment .Levels of miR143,IL-10,IFN-γin serum were assessed and calculated with the pearson coeffi-cient.PTC patients were assigned into miR 143 high and low expression group according to miR 143 expression , and IL-10 and IFN-γwere measured with routine protocol.Results：The relative expression of miR143 in paracancerous tissues and tumor tissues was （1.0± 0.15） vs （0.12±0.06）.miR143 was significantly associated with the survival rate and metastasis rate of patients （P〈0.001）.miR143 expression levels in IHH-4 pGenesil1-miR143 and control group were （ 8.63 ±0.71 ） vs （ 1.0 ±0.06 ） , and the proliferative index in pGenesil1-miR143 group was significantly lower than CON group （P〈0.01）.Percentage of Annexin and V＋positive cells in pGenesil1-miR143 and CON group was （40±7.2） vs （2±0.38）,and the ratio of the number of migrating cells was （40±7.2） vs （2±0.38） （P〈0.001）.Analysis showed that r2 were -0.4 and 0.62 for IL-10 and IFN-γ,respectively（P〈0.001）.In miR143 low group and high ex-pression group,expression levels of IFN-γwas （8±0.23） vs （11.2±0.12）,while IL-10 was （12±3.1） vs （8.43±0.44） （P〈0.05）. Conclusion：miR143 is low expresse

关 键 词：甲状腺乳头状瘤 miR143 免疫 IL-10 IFN-Γ 

分 类 号：R730.23[医药卫生—肿瘤]