卵巢癌组织中dbpA的表达及其对卵巢癌细胞增殖的影响  

Expression of DbpA Protein in Human Ovarian Cancer and Its Effects on Proliferation of Ovarian Cancer Cells

作　　者：刘晓英[1] 甘露[1] 张静[1] 樊阳阳 牛晶 

机构地区：[1]陕西省人民医院妇产科,陕西西安710069

出　　处：《现代生物医学进展》2017年第34期6637-6643,共7页Progress in Modern Biomedicine

基　　金：陕西省社会发展科技攻关项目(2016SF-181)

摘　　要：目的:探讨dbp A蛋白在卵巢癌组织中的表达及其对卵巢癌细胞增殖的影响。方法:通过荧光定量和蛋白质免疫印迹方法检测临床卵巢癌组织和正常癌旁组织中dbp A的表达量;设计合成针对dbp A基因的双链小干扰RNA转染人卵巢癌细胞系SKOV3和A2780细胞,用荧光定量和蛋白质免疫印迹方法检测细胞中dbp A的表达量,MTT法和克隆形成试验检测细胞的增殖能力和克隆形成能力,流式细胞术检测各组细胞周期和细胞凋亡的变化。结果:dbp A在卵巢癌组织、SKOV3和A2780细胞中表达较癌旁正常卵巢组织显著升高。沉默dbp A后,SKOV3和A2780细胞中dbp A蛋白表达量显著降低,SKOV3和A2780细胞增殖能力和克隆形成能力显著下降(P<0.05),G0/G1期细胞百分比显著增加(P<0.01),S期细胞百分比明显减少(P<0.05),细胞凋亡率显著升高(P<0.01)。结论:dbp A在卵巢癌组织和卵巢癌细胞SKOV3和A2780中过表达,沉默dbp A基因后可抑制SKOV3和A2780细胞的增殖能力和克隆形成能力。Objective： To investigate the expression of dbpA protein in human ovarian cancer and the effects of dbpA on proliferation of ovarian cancer cells. Methods： Expression of dbpA protein in ovarian cancer and noamal tissues was detected by real-time PCR and Western blot method; Chemically synthesized siRNA duplex targeting the dbpA gene was transiently transfected into human ovarian cancer cell line SKOV3 and A2780, expression of dbpA was detected by real-time PCR and Western blot; cell proliferation and cell cloning efficiency were determined by MTT experiment tests and limit dilution cloning method respectively; cell cycle and apoptosis was analyzed by flow cytometer. Results： In ovarian cancer tissue, SKOV3 cell and A2780 cell, the protein expression of dbpA quantity is higher than in normal ovarian tissues. In the dbpA gene silencing SKOV3 cells and A2780 cells, the expression of dbpA was significantly reduced, cell proliferation and cell cloning efficiency were significantly decreased （P〈0.05）, cells was increased in G0/G1 phase （P 〈0.01）, the percentage of S phase cells was significantly decreased （P 〈0.05） and the cells apoptosis rate was significantly increased （P 〈0.01）. Conclusion： DbpA was overexpressed in ovarian cancer tissue and ovarian cancer cells SKOV3 and A2780. SiRNA-mediated silencing of the dbpA gene in SKOV3 and A2780 could significantly reduce the ceils proliferation and cloning efficiency.

关 键 词：卵巢癌 DBPA 增殖 凋亡 

分 类 号：R-33[医药卫生] R737.31