电针预处理对PTSD模型大鼠焦虑样行为及海马Sirt1/MAO-A基因表达的影响  

The Influence of Electroacupuncture(EA) Preconditioning on Anxiety-like Behavior and the Expression of Sirt1/MAO-A in the Hippocampus of PTSD Rats

作　　者：李凤蕾 周翠红[2] 彭正午[2] 薛姗姗[2] 

机构地区：[1]哈励逊国际和平医院药学部,河北衡水053000 [2]第四军医大学西京医院心身科,陕西西安710032

出　　处：《现代生物医学进展》2017年第35期6839-6843,共5页Progress in Modern Biomedicine

基　　金：国家自然科学基金项目(81401109);陕西省科技新星资助项目(2015KTXX-51)

摘　　要：目的:探讨电针预处理对创伤后应激障碍大鼠(PTSD)焦虑样行为的改善作用及对海马去乙酰化酶Sirt1及单胺氧化酶MAO-A基因表达的影响。方法:将32只SD大鼠随机分为对照组(Sham),模型组(PTSD),电针刺激组(EA),电针预处理+模型(EA+PTSD),每组8只。电针刺激组(EA)和电针预处理+模型(EA+PTSD)组大鼠每天给予百会穴电针刺激(2/15 Hz,1 m A)30 min,连续刺激7 d。然后对模型组(PTSD)组和电针预处理+模型(EA+PTSD)组大鼠进行ESPS造模处理。造模结束14天后,通过旷场和高架十字测试其焦虑样行为,随后处死大鼠,通过实时定量PCR(RT-PCR)检测海马Sirt1和MAO-A的m RNA表达情况。结果:(1)PTSD大鼠具有明显的焦虑样行为,包括旷场中心的探索减少(44.94±13.66,16.86±7.12,P<0.05)和高架十字开臂运动时间减少(55.90±26.39,23.36±12.23,P<0.01),PTSD组大鼠海马Sirt1和MAO-A的m RNA表达增加(1.00±0.07,1.90±0.05;1.01±0.10,1.54±0.15,P<0.01)。(2)电针预处理可以缓解PTSD大鼠的焦虑样行为,PTSD与EA+PTSD组之间存在显著性差异(16.86±7.12,36.24±13.28,P<0.05;23.36±12.23,50.14±20.77,P<0.05)。(3)电针预处理可以抑制PTSD大鼠海马的Sirt1和MAO-A m RNA表达,PTSD与EA+PTSD组之间的Sirt1和MAO-A的m RNA水平存在显著性差异(1.90±0.05,1.30±0.08,P<0.05;1.54±0.15,1.23±0.15,P<0.05)。结论:电针预处理可以调节PTSD大鼠海马的Sirt1和MAO-A的m RNA水平,缓解PTSD大鼠的焦虑样行为。Objective： ,To investigate the effect of Electroacupuncture （EA） preconditioning on anxiety-like behavior and the effect on hippocampus Sirtl/MAO-A expression of PTSD rats. Methods： SD rats were randomly assigned to the following 4 groups （n=8 for each group）： Sham, PTSD, EA, EA ＋ PTSD. The EA and EA ＋ PTSD group received EA treatment at fi：equency of 2/15 Hz for 30 min daily for 7 days. Then, the PTSD and EA ＋ PTSD group rats received SPS＆SI After 14 days of treatment with SPS＆S, rats were experienced open field test and elevated plus maze test, then sacrificed and the mRNA level of Sirtl and MAO-A in the hippocampus were determined by real-time PCR. ResUlts： （1） The rat in PTSD group showed Significant less time in the central field（44.94± 13.66,16.86± 7.12, P〈0.05 ）and decreased rime＇in＇open arm （55.90± 26.39, 23.36±12.23, P〈0.01 ）, simultaneously mRNA level of Sirtl and MAO-A in hippocampus were increased（ 1.00±0.07, 1.90± 0.05; 1.01 ± 0.10, 1.54± 0.15, P〈0.01 ）; （2） Electroacupuncture preconditioning reversed anxiety behaviors in rats exposed to SPS＆S paradigm （16.86± 7.12, 36.24± 13.28, P〈0.05; 23.36± 12.23, 50.14± 20.77, P 〈 0.05 ）; （3） Electroacupuneture precondition restored the mRNA level of Sirtl and MAO-A in the hippocampus of PTSD rat（ 1.90± 0.05, 1.30 ±0.08, P〈0.05; 1.54 ±0.15, 1.23 ±0.15, P〈0.05）. Conclusion： Electroacupuncture preconditioning reversed anxiety behaviors in rats exposed to SPS＆S paradigm and restored the mRNA level of Sirtl and MAO-A in the hippocampus.

关 键 词：PTSD 电针 SIRT1 MAO-A 

分 类 号：R-33[医药卫生] R245.3