内皮素-1对肺组织磷酸胆碱二胞苷酰转移酶的影响  

Effects of endothelin-1 on phosphorylcholine cytidylyltransferase in cultured lung explants

作　　者：周伏文[1] 罗自强[1] 冯丹丹[1] 管茶香[1] 张长青[1] 秦晓群[1] 孙秀泓[1] 

机构地区：[1]中南大学湘雅医学院生理学教研室,长沙410078

出　　处：《中国药理学通报》2002年第4期382-385,共4页Chinese Pharmacological Bulletin

基　　金：国家自然科学基金资助课题 ;No 3 9770 2 84;湖南省中青年科技基金资助课题 ;No 99JZY2 0 76

摘　　要：目的　在离体肺组织培养模型上观察低浓度 (1×10 -13 ～ 1× 10 -9mol·L-1)内皮素 1(ET 1)对肺表面活性物质 (PS)脂质主要成分磷脂酰胆碱 (PC)合成的限速酶———CTP :磷酸胆碱二胞苷酰转移酶 (CCT)活性的影响。方法　采用无血清成年大鼠肺组织培养 ,以 [M 14 C]胆碱作为前体参入CDP 胆碱 ,液闪测定14 C放射性强度 ,反映CCT活性。结果　①CDP [M 14 C]胆碱的产量随微粒体和胞浆蛋白量增加、反应时间延长 (0～ 30min)而增多 ;②ET 1(1× 10 -12～ 1× 10 -9mol·L-1)以剂量依赖性提高微粒体CCT活性 ;③ET 1(1× 10 -10 mol·L-1)作用 8h ,微粒体CCT活性开始增强 ,随作用时间延长 (8～ 16h) ,酶活性逐渐增强 ;④ET 1(1× 10 -10 mol·L-1)可提高细胞微粒体CCT活性 ,而降低胞质CCT活性 ;⑤H 7和W 7均可分别抑制ET 1(1× 10 -10mol·L-1)促微粒体CCT活性增强的效应。结论　ET 1可促进肺组织细胞胞质CCT向微粒体转位 ,增加微粒体CCT活性 。AIM To investigate the effects of low concentrations (1×10 -13 ～1×10 -9 mol·L -1 ) endothelin 1 (ET 1) on the activity of CTP-phosphorylcholine cytidylyltransferase (CCT) from cultured lung explants without serum. METHODS CDP [ M 14 C] choline content as indicator of CCT activity was measured by liquid scintillation. RESULTS (1) The production of CDP [ M 14 C] choline from lung tissue microsomal CCT and cytosolic CCT was dependant on length of reaction time and the content of protein; (2) ET 1 increased lung tissue microsomal CCT activity in a dose dependent and time dependent manner; (3) ET 1 (1×10 -10 mol·L -1 ) enhanced lung tissue microsomal CCT activity and decreased cytosolic CCT activity; (4) The stimulatory effect of ET 1(1×10 -10 mol·L -1 ) on the microsomal CCT activity was abolished by protein kinase C (PKC) inhibitor H 7 and calmodulin antagonist W 7 respectively. CONCLUSION ET 1 at lower concentrations can activate CCT by translocation of enzyme from cytosol to microsomes and increase lung tissue microsomal CCT activity,which are mediated by PKC and calmodulin.

关 键 词：肺疾病 内皮素-1 肺组织 磷酸胆碱二胞苷酰转移酶 

分 类 号：R563[医药卫生—呼吸系统]