益气活血方(脑络欣通)对MCAO-R大鼠JAK-STAT信号转导通道的影响  

Effect of Supplementing Qi and Activating Blood Prescription(Naoluoxintong) on JAK-STAT Signal Transduction Pathway in MCAO-R Rats

作　　者：韩义皇[1] 王键[2] 

机构地区：[1]北京中医药大学,北京100029 [2]安徽中医药大学,安徽合肥230000

出　　处：《中医学报》2017年第12期2399-2403,共5页Acta Chinese Medicine

基　　金：国家科技支撑计划项目(2011BAZ03159)

摘　　要：目的:研究益气活血方(脑络欣通)对大脑中动脉阻塞再灌注(middle cerebral artery occlusion-reperfusion,MCAO-R)模型大鼠酪氨酸激酶信号转导子和转录激活子(janus kinase-signal transducer and activator of transcription,JAK-STAT)信号转导通道的作用机制与具体靶点。方法:将雄性SD大鼠随机分为假手术组、模型组、脑络欣通组,参照Koizumi和Longa法制备MCAO-R模型大鼠,通过神经功能缺损评分和TTC染色验证模型,通过基因芯片筛选大鼠海马组织JAK/STAT信号转导通路的差异基因,并通过Western blot蛋白电泳实验进行蛋白水平的验证。结果:经JAK/STAT信号转导通路PCR基因芯片检测,按倍数变化(Fold change,Fc)>1.5标准,从该通路89个基因中筛选出6个主要差异基因,分别是Fcgr1α、Ifngr1、IL105、PRLR、Scocs1、Socs5;经过PCR基因芯片检测,与模型组大鼠比较,脑络欣通组大鼠Socs5 mRNA明显增加(F值为1.62,P<0.05);经过Western Blot蛋白电泳实验验证,与模型组大鼠比较,脑络欣通组大鼠PRLR蛋白明显减少(F值为6.161,P<0.05)。结论:益气活血法通过促进模型大鼠海马Socs5和Socs1 mRNA表达,抑制模型大鼠PRLR蛋白表达,进而抑制JAK/STAT通路的表达,从而促进NSCs向神经元分化,抑制神经细胞的凋亡,促进脑卒中后中枢神经系统功能恢复,对脑卒中后的神经损伤现象起保护作用。Objective： To study the effects of Naoluoxintong on middle cerebral artery occlusion-reperfusion（ MCAO-R） model rats Janus kinase-signal transducer and activator of transcription（ JAK-STAT） signal transduction pathways mechanism and specific targets. Methods： Male SD rats were randomly divided into sham operation group,model group,Naoluo xintong group,MCAO-R model rats with Koizumi and Longa staining method,neural function defect score and TTC staining verification model,and the differential gene of JAK/STAT signal transduction pathway in rat hippocampus was screened by gene chip. The protein level was tested by Western blot protein electrophoresis. Results： According to the JAK/STAT signal transduction pathway PCR gene chip detection,according to the multiple variation（ Fold change,Fc） 1. 5 standard,6 main differential genes were screened out from the 89 genes of the pathway,namely Fcgr1,Ifngr1,IL105,PRLR,Scocs1,and IL105. After PCR gene chip detection,compared with themodel group,the Socs5 mRNA of the rats in the group of Naoxintong group increased significantly（ F value was 1. 62,P〈0. 05）.The Western Blot protein electrophoresis test showed that the PRLR protein of the rats in Nao xintong group was significantly reduced（ F value was 6. 161,P〈0. 05） compared with the model rats. Conclusion： Supplementing Qi and activating blood circulation method can promote the expression of hippocampus in rat model of Socs5 Socs1 and mRNA,inhibit the expression of PRLR protein in rat model,and then inhibit the expression of JAK/STAT pathway,thereby promoting the differentiation of NSCs into neurons,inhibition the apoptosis of nerve cells,promoting function recovery of central nervous system after cerebral apoplexy,and protective effect on neural phenomenon after stroke injury.

关 键 词：益气活血方 脑络欣通 中动脉阻塞再灌注 脑卒中 JAK-STAT 大鼠 

分 类 号：R285.5[医药卫生—中药学]