PD-L1在人胶质瘤中的表达及其对肿瘤内浸润性T细胞凋亡的影响  

Expression of programmed death ligand-1 in human glioma and its influence to intratumor infiltrating T lymphocyte apoptosis

作　　者：李巧转[1] 李娴[2] 

机构地区：[1]解放军第101医院病理科,江苏无锡214044 [2]重庆医科大学病理教研室,400016

出　　处：《重庆医学》2018年第1期37-39,42,共4页Chongqing medicine

基　　金：江苏大学医学临床科技发展基金项目(JLY20160200)

摘　　要：目的探讨程序性死亡-配体1(PD-L1)在人胶质瘤组织中的表达及其对肿瘤内浸润性T细胞凋亡的影响。方法对2007-2011年解放军第101医院收治的80例胶质瘤患者肿瘤组织及18例正常脑组织进行PD-L1免疫组织化学方法检测,同时检测程序性死亡受体-1(PD-1)在胶质瘤浸润性T淋巴细胞的表达,进行相关性分析,TUNEL法检测胶质瘤内浸润性淋巴细胞的凋亡,应用流式细胞术分析PD-L1促进胶质瘤患者外周血中活化T细胞的凋亡情况。结果正常脑组织不表达PD-L1,PD-L1在胶质瘤中的阳性表达率为42.50%(34/80),与胶质瘤病理分级、坏死和预后相关(P<0.05)。部分胶质瘤组织及浸润T淋巴细胞表达PD-1,且PD-L1阳性的胶质瘤病例淋巴细胞凋亡表达较PD-L1阴性病例明显增高。PD-L1组患者外周血活化T细胞的凋亡率为42.55%明显高于空白对照组,加入抗PD-1阻断PD-L1与T淋巴细胞结合后,PD-L1+抗PD-1组活化T淋巴细胞凋亡率下降到26.80%。结论人胶质瘤异常高表达PD-L1并与PD-1结合促进淋巴细胞的凋亡。Objective To investigate the expression of programmed death ligand-l（PD-L1） in glioma and its influence to in- tratumor infiltrating T lymphocyte apoptosis. Methods PD-L1 in 18 cases of normal brain tissues and 80 cases of glioma tissues treated in this hospital during 2007 to 2011 was determined by immunohistochemistry staining, meanwhile the PD-L1 expression in glioma infiltrating T lymphocyte was determined. The correlation analysis was performed. The apoptosis of intratumor infiltrating lymphocytes was examined by TUNEL assay;the peripheral blood activated T lymphocyte apoptosis promoted by PD-L1 was analyzed by flow cytometry. Results No expression of PD-L1 in normal brain tissues was observed,but the positive expresston rate in glioma was 42.50%（34/80）,which was correlated with pathological grade,necrosis and prognosis（P〈0.05）. PD-1 was expressed in part of tumor infiltrating T lymphocytes and glioma tissues,moreover the lymphocyte apoptosis expression in the cases of PD-L1 positive glioma was significantly increased compared with that in the cases of PD-L1 negative glioma. The apoptosis rate of activated T lymphocytes was 42.55% respectively,which was significantly higher than that in the blank group; however, adding anti-PD-1 for bloc-king the combining of PD-L1 with T lymphocyte,the lymphocytes apoptotic rate in the PD-L1- anti-PD-1 group was dropped to 26.80%. Conclusion PD-Llhas aberrantly high expression in human gliorna and is combined with PD-1 to promote the apoptosis of lymphocyte.

关 键 词：神经胶质瘤 细胞凋亡 程序性死亡受体-1 程序性死亡-配体1 

分 类 号：R739.4[医药卫生—肿瘤]