癫痫持续状态模型中高迁移率族蛋白1、N-甲基-D-天冬氨酸受体2B亚单位的表达  

Expression of high-mobility group box- ! and N-methyl-D-aspartate receptor in status epilepticus

作　　者：吴丹 韩笑 冯靖[3] 宋毅军[2] 

机构地区：[1]天津医科大学宝坻临床学院神经内科,301800 [2]天津医科大学总医院神经内科神经病学研究所 [3]天津医科大学总医院呼吸内科 [4]天津市蓟州区人民医院神经内科

出　　处：《中华医学杂志》2018年第1期51-55,共5页National Medical Journal of China

基　　金：国家自然科学基金资助项目（81270144,81570084）;天津市应用基础与前沿技术研究计划（14JCZDJC35400）;天津市“131”创新型人才团队

摘　　要：目的观察癫痫持续状态细胞模型及动物模型的高迁移率族蛋白1（HMGB1）、N-甲基-D-天冬氨酸（NMDA）受体2B亚单位的表达变化。方法（1）孕16～18d的sD大鼠，取其胎鼠海马神经元培养至第7天，应用无镁细胞外液处理3h后制备Sombati癫痫细胞模型，将细胞培养皿编号后采用随机数字表法分为Sombati癫痫神经元组、Sombati癫痫神经元间歇低氧后正常氧培养组。采用real-timePCR检测两组胎鼠海马神经元HMGB1、NR2BmRNA。（2）选取体质量相近的SD大鼠，鼠编号后采用随机数字表法分为正常对照组（Ns组）和LI-PILO组；LI-PILO组采用LI-PtLO腹腔注射诱导大鼠癫痫持续状态模型，采用real-timePCR检测两组SD大鼠海马神经元HMGB1、NR2BmRNA。结果Sombati癫痫神经元间歇低氧后正常氧培养2、4、6h时，HMGB1mRNA相对表达量分别为0．00501±0．00054、0．02676±0．00375、0．00352±0．00033，与同一时间点Sombati癫痫神经元组HMGB1mRNA相对表达量相比，均P〈0．05；Sombati癫痫神经元间歇低氧后正常氧培养2、4、6h时，NR2BmRNA相对表达量分别为0．00884±0．00069、0．01223±0．00090、0．02911±0．00071，与同一时间点Sombati癫痫神经元组NR2BmRNA相对表达量相比，均P〈0．05。LI—PILO组在造模成功后6、8、10hHMGB1mRNA相对表达量分别为0．000114-0．00009、0．00018±0．00001、0．00011±0．00001，与同一时间点NS组HMGB1mRNA相对表达量相比，均P〈0．05；LI-PILD组在造模成功后6、8、10hNR2BmRNA相对表达量分别为0．19612±0．00941、0．23211±0．00627、0．27248±0．00584，与同一时间点Ns组NR2BmRNA相对表达量相比，均P〈0．05。结论Sombati癫痫神经元间歇低氧后正常氧培养2、4hHMGB1mRNA相对表达量升高，6h表达降低；LI-PILO组在造模成功后6、8hHMGB1mRNA相对表达量升高，10h相对表达量降低；Sombati癫痫神经元间歇低氧后正常氧培养组及LI-PILO组NR2BmRNA相对�Objective To investigate the expression changes of high mobility group box-1 （HMGB1） and the 2B receptor of N- methyl -D- aspartate receptor （NR2B） in status epilepticus （SE）. Methods （ l ） Primary hippocampal neurons from SD rats with 16 to 18 days of fetal age were cultured in vitro for 7 days, and exposed to Mg2 free media for 3 hours. Those cultured neurons were randomly divided into control group and intermittent hypoxia group. （2） SD rats with similar weight were selected and randomly divided into control group and SE model group. The rat model with SE was established by an intraperitoneal injection of lithium chloride-piloearpine （LI-PILO）. Real-time PCR technique was used to detect the expression of HMGB1 and NR2B mRNA. Results In Sombati＇s cell model cultured in normal concentration of oxygen, the HMGB1 mRNA expression levels were 0. 005 01 ± 0. 000 54,0. 026 76 ± 0. 003 75,0. 003 52 ± 0. 000 33, and the NR2B mRNA expression levels were 0. 008 84 ± 0. 000 69, 0. 012 23 ±0. 000 90,0.029 11±0.000 71, respectively, at 2, 4 and 6 h; compared with the expressions of HMGB1 and NR2B mRNA at the same time points of Sombatis cell model groups, the differences were also significant （ all P 〈 0. 05 ）. After the successful establishment of epilepsy model, the HMGB1 mRNA expression levels were 0.000 11 ±0. 000 09,0. 000 18 ±0. 000 01,0. 000 11±0. 000 01, and the NR2B mRNA expression levels were 0. 196 12 ± 0. 009 41,0. 232 11 ± 0. 006 27,0. 272 48± 0. 005 84, respectively, at 6, 8 and 10 h; compared with the expressions of HMGB1 and NR2B mRNA at the same time points of control groups, the differences were all significant （ all P 〈 0.05）. Conclusion HMGB1 mRNA expression levels increase at 2, 4 h, decrease at 6 h in the Sombati＇s cell model in normal oxygen culture, while increase at 6, 8 h, and decrease at 10 h in LI-PILO induced rat model with SE ; the NR2B mRNA relative expression increases with time in both the Sombati＇s cell model in normal oxygen culture and

关 键 词：癫痫持续状态 细胞低氧 海马神经元 高迁移率族蛋白1 N-甲基-D-天冬 氨酸受体 

分 类 号：R742.1[医药卫生—神经病学与精神病学]