CRISPR/Cas9介导的基因组编辑技术及研究进展与应用  

Progress in Researches of CRISPR/Cas9 Mediated Plant Genome Editing

作　　者：邢国杰[1] 李桐 郑慧瑾 张玲[1] 姜晓坤[2] 

机构地区：[1]吉林省农业科学院农业生物技术研究所,长春130033 [2]吉林农业科技学院,吉林吉林132101

出　　处：《东北农业科学》2017年第6期28-30,35,共4页Journal of Northeast Agricultural Sciences

基　　金：吉林省农业科学院自由创新工程项目(CXGC2017ZY024)

摘　　要：CRISPR/Cas系统是一种从细菌和古生菌中衍生而来的适应性免疫系统,当细菌遭受病毒或者外源质粒入侵时,会产生相应的"记忆",从而防止外来DNA片段的侵袭。CRISPR/Cas9系统以设计操纵简便、编辑高效与通用性广等优势已经成为改良作物的新一代基因组编辑技术。本文从CRISPR/Cas9介导的基因组编辑技术的发展与应用等方面综述其最新研究进展,并着重介绍该技术的关键影响因素,为相关研究者提供参考。CRISPR/Cas9 system is an immune system of prokaryotes, which is used to resist invasion of exogenous genetic material. When bacteria are attacked by viruses or exogenous plasmids, they will generate corresponding memories, so that they can resist their invasion. The CRISPR/Cas9 system has become a new generation of genome editing technology with the advantages of simple design, efficient editing and wide versatility. The latest research progress from the development and application of genome editing technology mediated by CRISPR/Cas9 was re- viewed in the paper which focused on the key influencing factors of the technology, so as to provide references for relevant researchers.

关 键 词：CRISPR/Cas9 基因编辑 植物 

分 类 号：Q789[生物学—分子生物学]