DNMT1和CCNA1在不同级别宫颈病变组织中的表达及临床意义  

Expression and clinical significance of DNMT1 and CCNA1 in different grades of cervical lesions

作　　者：曹佳宇 白洁 董建新 邵婷 陈燕 

机构地区：[1]华北理工大学基础医学院、河北省慢性疾病重点实验室、唐山市慢性病临床基础研究重点实验室,河北省唐山市063000 [2]华北理工大学附属医院妇产科

出　　处：《实用医学杂志》2017年第24期4105-4109,共5页The Journal of Practical Medicine

基　　金：河北省医学科学研究重点课题计划项目(编号:20160726)

摘　　要：目的探讨DNMT1和CCNA1在不同级别宫颈病变组织中的表达及临床意义。方法选取病理学诊断的正常宫颈(NC)、宫颈低级别上皮内病变(LSIL)、宫颈高级别上皮内病变(HSIL)、宫颈鳞状细胞癌(SCC)组织各30例,采用Western blot法、实时荧光聚合酶链反应(q RT-PCR)法检测各组DNMT1、CCNA1 mRNA和蛋白的表达。结果 DNMT1 mRNA和蛋白表达量在LSIL、HSIL及SCC组织中均高于NC组织(F=117.93,P<0.05;F=61.24,P<0.05),随着病变程度的增加,DNMT1 mRNA和蛋白的表达水平逐渐上升(χ~2趋势=26.25,P<0.05;χ~2趋势=26.60,P<0.05)。CCNA1 mRNA和蛋白的表达水平在HSIL、SCC组织中均低于NC、LSIL组织(F=77.04,P<0.05;F=57.15,P<0.05),随着病变程度的增加,CCNA1 mRNA和蛋白表达量逐渐下降(χ~2趋势=64.19,P<0.05;χ~2趋势=60.24,P<0.05)。DNMT1、CCNA1 mRNA和蛋白在LSIL、HSIL、SCC组织中的表达呈负相关(r=-0.75、-0.56,P<0.05)。结论 DNMT1 mRNA、蛋白的高表达,CCNA1 mRNA、蛋白低表达,二者可能与宫颈癌的发生发展有关。Objective To investigate the expression and clinical significance of DNMT1 and CCNA1 in different grades of cervical lesions. Methods Cervical tissues were selected from normal cervical（NC）,low-grade cervical intraepithelial lesion（LSIL）,high-grade cervical intraepithelial lesion（HSIL）and cervical squa-mous cell carcinoma（SCC）from each thirty patients.The expression of DNMT1 and CCNA1 mRNA and protein was examined by Western Blot analysis and qRT-PCR in cervical tissues. Results The expression of DNMT1 mRNA and protein in LSIL,HSIL and SCC was higher than in NC（F=117.93,P〈0.05;F=61.24,P〈0.05）. Expression of DNMT1 mRNA and protein was increased steadily according to severity of cervical lesions（χ2trend=26.25,P〈0.05;χ2trend=26.60,P〈0.05）.The expression of CCNA1 mRNA and protein in HSIL and SCC was lower than that in NC and LSIL（F = 77.04,P 〈 0.05;F = 57.15,P 〈 0.05）. Expression of CCNA1 mRNA and protein was decreased steadily according to severity of cervical lesions（χ2trend=64.19,P〈0.05;χ2trend=60.24,P〈0.05）. There was a negative correlation between expression of DNMT1 and CCNA1 protein in LSIL,HSIL,SCC （r=-0.75,-0.56,P〈0.05）. Conclusion In DNMT1 mRNA there is high expression of protein and in CCNA1 mRNA there is low expression of protein,but both may be related to the occurrence and development of cervical cancer.

关 键 词：DNA甲基转移酶1 细胞周期蛋白A1 宫颈癌 宫颈癌前病变 

分 类 号：R737.33[医药卫生—肿瘤]