SPOP蛋白在胃癌中的表达及其临床意义  

Expression and prognostic analysis of SPOP protein in gastric carcinoma

作　　者：徐骏飞[1] 

机构地区：[1]南通大学附属医院普通外科,江苏226001

出　　处：《交通医学》2017年第5期409-412,共4页Medical Journal of Communications

基　　金：南通市卫计委青年科研基金项目(WQ2016068)

摘　　要：目的:探讨SPOP蛋白在胃癌组织中的表达及其临床意义。方法:使用Western blot方法和实时定量聚合酶链反应(Real-time PCR)法检测60例胃癌组织及癌旁组织中SPOP蛋白和mRNA表达,采用Pearsonχ~2检验胃癌中SPOP表达与临床病理学特征的相关性,采用Kaplan-Meier法分析SPOP表达与胃癌患者生存期的关系,Cox回归分析各因素与生存期的关系。结果:胃癌组织SPOP蛋白及mRNA表达水平明显低于癌旁组织,差异具有统计学意义(P<0.05)。SPOP表达与胃癌TNM分期(P=0.038)、分化程度(P=0.029)、浸润深度(P=0.009)、淋巴结转移(P=0.001)相关,但与患者性别、年龄、肿瘤大小以及是否远处转移无关。Cox比例风险模型分析发现,SPOP表达(P=0.002)、TNM分期(P=0.018)及分化程度(P=0.010)是影响胃癌生存预后的独立因素;SPOP高表达组患者的生存率高于低表达组,差异有统计学意义(P=0.036)。结论:SPOP可能参与胃癌的发生发展,是胃癌独立的预后影响因素。Objective：To investigate the clinicopatholo gical and prognostic value of SPOP expression in gastric cancer.Methods：Western blot and real-time quantitative PCR were employed to detect the expression level of SPOP in gastric cancer tissues of 60 patients.Correlation between variables was analyzed by Pearson χ~2 method.Moreover,the Kaplan-Meier method and log-rank test were employed to compare the overall survival between the SPOP low expression group and the SPOP high expression group.The correlation of survival with clinicopathological parameters,including SPOP level,was investigated with multivariate analyses.Results：There was significant correlation between SPOP expression and TNM stage（P=0.038）,Tumor differentiation（P=0.029）,Depth of invasion（P=0.009） and Lymph node metastasis（P=0.001）,but there was no significant correlation between SPOP expression and gender,age,Tumor size and Distant metastasis.Moreover,a multivariate analysis was performed to evaluate the independent prognostic roles of SPOP after adjusting for other significant covariates.All variables that significantly affected the survival in univariate analysis were introduced into a Cox proportional-hazard model.It was found that TNM stage（P=0.018）,histological differentiation（P=0.010） and SPOP expression（P=0.002） influenced the overall survival in gastric cancer patients.Kaplan-Meier survival analysis showed that low SPOP expression exhibited a significant correlation with poor prognosis for gastric cancer patients（P=0.036）.Conclusion：SPOP expression was a significant independent prognostic factor of gastric cancer patients.

关 键 词：胃癌 SPOP蛋白 复发 预后 

分 类 号：R735.2[医药卫生—肿瘤]