微小RNA-25在肺癌患者中的临床诊断和应用价值  

Clinical diagnosis and application of microRNA-25 in lung cancer patients

作　　者：张强[1] 曹磊[1] 钟健[1] 黎增亮 

机构地区：[1]南京市胸科医院心胸外科,南京2100000

出　　处：《癌症进展》2017年第11期1302-1304,共3页Oncology Progress

摘　　要：目的探索血清微小RNA-25(miRNA-25)在肺癌中的表达差异,研究其在肺癌早期诊断中的应用价值,为肺癌的早期诊断提供一定的参考依据。方法收集160例肺部疾病患者资料,根据患者的影像学检查和病理学检查等资料确诊为肺癌的患者共80例作为肺癌组,同时选取同期收治的肺部良性疾病患者80例作为对照组。通过医院门诊或住院系统收集研究对象的年龄、性别、体重指数(BMI)等一般人口学特征资料,并对所有患者进行实验室检测miRNA-25。采用ROC曲线对资料进行数据分析。结果对于对照组和肺癌组miRNA-25表达的ROC分析,截断点为4.12,曲线下面积为0.742,灵敏度为62.2%,特异度为89.8%;对于对照组和非小细胞肺癌组,截断点为2.21,曲线下面积为0.721,灵敏度为66.7%,特异度为72.8%;对于对照组和小细胞肺癌组,截断点为5.46,曲线下面积为0.789,灵敏度为64.5%,特异度为94.2%。结论 miRNA-25在肺癌患者中的表达相比肺部良性疾病患者有明显的升高,尤其是在小细胞肺癌的表达中,对于肺癌患者的早期发现和预后治疗具有重要的参考价值。Objective To explore the difference of serum micro RNA-25（miRNA-25） expression in lung cancer and to study its application in the early diagnosis to help improve the early diagnosis. Method A total of 160 patients with lung diseases were included in the analysis, in which 80 cases who were diagnosed as lung cancer by imaging and pathological examinations were identified as lung cancer group, while another 80 cases with benign lung diseases were assigned as control group. The general demographic characteristics such as age, sex, and BMI were collected by hospital outpatient or inpatient system, and laboratory tests were performed for all subjects to detect miRNA-25. Data were analyzed by ROC curve. Result For the analysis of miRNA-25 expression in control group and lung cancer group, the cutoff point was 4.12, AUC was 0.742, with sensitivity being 62.2%, specificity being 89.8%; for control group and nonsmall cell lung cancer group, the cut-off point was 2.21, AUC was 0.721, with sensitivity being 66.7% and specificity being 72.8%; for control group and small cell lung cancer group, the cut-off point was 5.46, AUC was 0.789, with sensitivity being 64.5%, and specificity being 94.2%. Conclusion The expression of miRNA-25 is significantly higher in lung cancer patients, especially in those with non-small cell lung cancer, than in those with benign lung diseases, and is of great value for the early detection and prognostic treatment of lung cancer patients.

关 键 词：肺癌 血清微小RNA-25 诊断价值 

分 类 号：R734.2[医药卫生—肿瘤]