新疆伊犁地区尼勒克县人群泡球蚴感染病例调查及nad2、cob基因片段分析  

Emerging alveolar echinococcosis and gene fragments of Nad2 and Cob in Nileke County,Yili area of Xinjiang,China

作　　者：郭永忠 初伊明 阿尔新·卡德尔 郭宝平 周璨林 李军 张文宝 郭刚 

机构地区：[1]新疆伊犁哈萨克自治州友谊医院,伊宁市835000 [2]新疆医科大学第一附属医院临床医学研究院,新疆重大疾病医学重点实验室-省部共建国家重点实验室培育基地,乌鲁木齐830054

出　　处：《中国人兽共患病学报》2017年第12期1066-1070,共5页Chinese Journal of Zoonoses

基　　金：国家自然科学基金项目(No.U1303203,No.31760734)~~

摘　　要：目的了解新疆伊犁地区尼勒克县泡球蚴感染确诊病例人群分布及病原遗传特征。方法收集和调查尼勒克县境内人群泡球蚴感染病例相关数据,并利用分子生物学技术对收集到的患者病灶肝组织样本,针对线粒体nad2和cob基因片段开展病原分子检测和分析。结果截止2015年底共收集到泡球蚴感染患者资料共计48例,首例感染病人确诊于1989年。近5年确诊病例占到所有病例数的45.8%,年均确诊率为1.7/10万。感染人群沿喀什河流域两岸分布,在乌拉斯台乡尤为集中。患病人群中35-44岁占到38.6%,男性占64.6%,农牧民占95.8%。通过对11份病例样本检测nad2、cob基因片段进一步证实为泡球蚴感染,序列自身比对完全同源。结论泡球蚴在尼勒克县属于新发传染病。包括尼勒克县在内的伊犁河谷地带为泡球蚴的自然疫源地,该县境内的泡球蚴病原核酸序列较为保守单一。We investigated the endemic situation of alveolar echinococcosis(AE)in Nileke County,Yili Prefecture,Xinjiang and its genetic diversity of Echinococcus multilocularis.Human AE cases in the county were retrospectively investigated including their location and surrounding ecological conditions.Sequence detection and analysis of nad2,cob gene fragments of AE mtDNA from patients was used to identify genotype variation.Results showed that a total of 48 AE cases were diagnosed and the first AE patient was identified in 1989 in the county.The 45.8% of AE cases were found in the recent 5 years(2011-2015)and annual prevalence was 1.7 per 100 000.The patients were distributed along the Kashgar River,particularly intensive in Wulasitai Township.The 38.6% of the patients were aged arranged 35-44 years old,64.6% were male and 95.8% were farmers and herdsmen.AE cases were confirmed further and there was only one haplotype by sequencing analysis from 11 AE clinical patients in the county.It suggests that Nileke County is AE foci,and alveolar echinococcosis with sequences conserved is an emerging disease in the county.

关 键 词：泡球蚴病 多房棘球蚴病 基因变异 尼勒克县 

分 类 号：R383.3[医药卫生—医学寄生虫学]