基于RFLP-PCR的猪肉溯源技术研究  

Study on the traceability technology of pork based on RFLP-PCR

作　　者：吴华莉[1,2] 涂尾龙 曹建国[1,2] 吴潇 常华[4] 都启晶 谈永松[1,2] 

机构地区：[1]上海市农业科学院畜牧兽医研究所,上海201106 [2]上海种猪工程技术研究中心,上海201302 [3]上海市农业科学院生物技术研究所,上海201106 [4]云南农业大学动物科学技术学院,昆明650201 [5]青岛农业大学食品科学与工程学院,青岛266109

出　　处：《上海农业学报》2017年第6期57-62,共6页Acta Agriculturae Shanghai

基　　金：上海市科委科技支撑项目(13391900100);上海市科技兴农重点攻关项目[沪农科攻字(2013)第5-6号);沪农青字(2014)第1-34号]

摘　　要：选取杜洛克、大白猪和长白猪等3个品种共330头样品,提取基因组DNA,采用RFLP-PCR方法检测13个基因在3个猪品种群体内15个SNP位点的多态性,以期寻找到多态性信息含量丰富的SNP位点用于猪肉DNA溯源标记。结果表明:ADAMTS、DAZL、FBXO32、FUT1、MC4R、MyoG、NR4A1和PSMB基因的9个SNP位点可以用于杜洛克猪、大白猪和长白猪肉产品检测。根据9个SNP位点基因型对应的9个数字和字母组合形成的DNA条形码可以用于杜洛克猪、大白猪和长白猪肉产品溯源。A total of 330 samples of Duroc,Large white and Landrace pigs were selected to extract genome DNA. The RFLP-PCR method was used to detect the polymorphism of 13 genes in 15 SNP loci in 3 pig varieties to find SNP loci with rich polymorphism information for the traceability marker of pork DNA. The results showed that 9 SNP loci ADAMTS,DAZL,FBX032,FUT1,MC4 R,MyoG,NR4 A1 and PSMB genes could be used for the detection of Duroc, Large white and Landrace pork products. DNA barcodes formed by the combination of 9 numbers and alphabetical combinations of 9 SNP loci genotypes could be used in Duroc and Large white,Landrace pork traceability.

关 键 词：猪肉 DNA溯源 SNP 

分 类 号：S879.2[农业科学—畜牧兽医]