Van-clear替代二甲苯对乳腺癌Her-2蛋白表达的影响  

Effects of Van-clear substituting xylene on HER2 protein expression in breast cancer

作　　者：陈志强 王莹 米贤军 陈昂 黄华勇 徐秀梅 代新珍 邓文同 段立锋 

机构地区：[1]南方医科大学附属中山博爱医院病理科,广东中山528400

出　　处：《诊断病理学杂志》2017年第12期918-921,共4页Chinese Journal of Diagnostic Pathology

基　　金：广东省医学科学技术研究基金立项课题(A2017321);广东省中山市卫生和计划生育局医学科研立项课题(2017J111);广东省中山市卫生和计划生育局医学科研立项课题(2014J128)

摘　　要：目的比较环保透明脱蜡液Van-clear和二甲苯在免疫组织化学法检测乳腺癌Her-2蛋白表达的差异,探讨Van-clear替代二甲苯的可行性。方法收集中山市博爱医院2013-06—2017-03间住院部送检的乳腺浸润性导管癌标本95例,同一肿瘤病变部位切取2个样本,用抽签法随机分为A、B组。A组采用二甲苯透明脱蜡制作切片95张;B采用Van-clear透明脱蜡制作切片95张。观察生物显微镜下Her-2免疫组织化学染色大体情况,采用免疫组织化学法检测Her-2蛋白表达情况,SPSS20.0比较两组间蛋白阳性率的差异。结果生物显微镜下,A、B两组切片Her-2免疫组织化学染色结果:背景清晰、阳性着色定位准确(胞膜)、阳性强度可靠、呈色鲜明,无非特异性染色产生,阴性、阳性对照染色结果可靠。(2)A、B两组切片Her-2蛋白阳性率分别为24.2%、26.3%,两组间阳性率差异无统计学意义(χ2=0.50,P>0.05),且Her-2蛋白表达符合率为97.9%。结论环保透明脱蜡液Van-clear有潜能替代传统试剂二甲苯应用于免疫组织化学法检测乳腺癌Her-2蛋白表达。Objective To compare the differences in the expression of HER2 protein in breast cancer between the environment-friendly transparent deparoffining solution Van-clear and xylene in immunohistochemistry,and to explore the feasibility of Van-clear instead of xylene. Methods 95 cases of breast invasive ductal carcinoma specimens were collected from the Zhongshan Boai Hosipital from June 2013 to March 2017. Two samples were taken from the same lesion site,and they were randomly divided into 2 groups,named Groups A and B. Xylene deparoffining was used in Group A,while Van-clear transparent deparoffining in Group B to make 95 slices. The general situation of the HER2 immunohistochemical staining was observed under the biological microscope,the expression of HER2 protein was detected by immunohistochemistry,and the difference of protein positive rate was compared between the two groups by SPSS20. 0.Results Under the biological microscope,the results of the HER2 immunohistochemical staining in Groups A and B were as following： the background was clear,the positive staining was accurate（ in the cell membrane）,the positive intensity was reliable,the color was bright,there was no nonspecific staining,and the negative and positive control staining results were reliable. The positive rates of HER2 protein in Groups A and B were 24. 21% and 26. 32%,respectively. There was no significant difference in the positive rate between these two groups（ χ2= 0. 50,P 〉 0. 05）. Besides,the coincidence rate of HER2 protein expression was 97. 89%. Conclusion Environmental protection transparent deparoffining solution Van-clear has the potency to replace the traditional reagent xylene in the detection of HER2 protein expression in breast cancer by immunohistochemistry.

关 键 词：环保透明脱蜡液Van-clear 二甲苯 乳腺浸润性导管癌 免疫组化 HE染色 

分 类 号：R737.9[医药卫生—肿瘤]