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作 者:汪洲[1] 许鹏飞 肖玲玲[2] 王栋 龚大春[1]
机构地区:[1]三峡大学生物催化重点实验室,湖北宜昌443002 [2]湖北三峡职业技术学院,湖北宜昌443002 [3]车用生物燃料技术国家重点实验室,河南南阳473000
出 处:《三峡大学学报(自然科学版)》2018年第1期109-112,共4页Journal of China Three Gorges University:Natural Sciences
基 金:宜昌市科技攻关项目:纤维素糖化制备微生物柴油的工艺研究(项目编号:A15-301-b04)
摘 要:研究了醋酸泼尼松对皮状丝孢酵母生长和油脂积累的影响.结果表明:添加醋酸泼尼松100mg/L,可以有效促进皮状丝孢酵母的生长,使皮状丝孢酵母生物量达到最大,比未添加醋酸泼尼松提高68%;一次添加醋酸泼尼松200mg/L,对皮状丝孢酵母油脂积累油脂最为明显,可以提高98%的产量;如果采用分批添加醋酸泼尼松200mg/L,即30h前使添加醋酸泼尼松维持100mg/L,30h后补加醋酸泼尼松,维持在200mg/L,微生物油脂产量可以从最初2.5g/L提高到5.7g/L,产量提高128%;通过气相检测发现醋酸泼尼松的添加对酵母油脂脂肪酸的组成含量也有影响,油酸、亚油酸含量分别增加21.6%、32.3%.本文为提高皮状丝孢酵母生物量和油脂积累提供了一种新的方法.The effect of prednisone acetate (PA) on the productions of microbial oils from Trichosporon cuta- neum was investigated. The results showed that PA can effectively improve the growth of Trichosporon cutaneum and the biomass could reach the maximum with the addition of 100 mg/L PA, which was significantly increased by 68%. Moreover, the accumulation of fatty acids was observed to be up to the 98% in production with one-time addition of 200 mg/L PA. Furthermore, the products of microbial oils could reach 5.7 g/L from 2.5 g/L with 128% increasing, which was achieved by step-addition of 200mg/L PA maintaining 100 mg/L concentration within 30 hours and 200mg/L concentration after 30 hours. The compositions of the fatty acids were analyzed and the contents of oleic acid and linoleic acid were increased 21.6 and 32.3%, respectively. This study provided a new method for the improvement of the biomass and oil accumulation in the depth ex- ploration of oleaginous yeast Trichosporon cutaneum.
分 类 号:TQ925[轻工技术与工程—发酵工程]
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