乙醛脱氢酶1和转化生长因子-β2在三阴性乳腺癌中的表达及临床意义  

Expressions and clinical, significances of aldehyde dehydrogenase 1 and transforming growth factor-β2 in triple negative breast cancer

作　　者：郭崇勇[1] 李永梅[2] 李柏成[3] 周凌[4] 张建[3] 贾宗师[1] 

机构地区：[1]山东省滨州市人民医院乳腺外科(普外三科),256600 [2]山东省滨州市人民医院妇科,256600 [3]山东省滨州市人民医院病理科,256600 [4]上海市第一人民医院分院普外科

出　　处：《国际肿瘤学杂志》2017年第11期801-805,共5页Journal of International Oncology

基　　金：山东省自然科学基金（ZR2014HP036）

摘　　要：目的 探讨乳腺肿瘤干细胞标志物乙醛脱氢酶1（ALDH1）和转化生长因子-β2（TGF-β2）在三阴性乳腺癌中的表达及其临床意义。方法 采用免疫组织化学染色法检测60例三阴性乳腺癌患者肿瘤组织中ALDH1和TGF-β2蛋白的表达，并进行相关性分析和无瘤生存期、总生存期分析。结果 在60例乳腺癌原发灶中，ALDH1蛋白和TGFβ2蛋白阳性表达分别为23例（38.33%）和38例（63.33%）。ALDH1蛋白在乳腺癌组织中的阳性表达与肿瘤大小（χ2=0.307，P=0.580）、组织学分级（χ2=4.244，P=0.120）、临床分期（χ2=0.982，P=0.612）以及淋巴结转移（χ2=1.111，P=0.292）均无关。TGFβ2蛋白在乳腺癌组织中的阳性表达与组织学分级（χ2=4.651，P=0.098）、淋巴结转移（χ2=3.513，P=0.061）、临床分期（χ2=1.310，P=0.519）和肿瘤大小（χ2=0.629，P=0.428）无关。ALDH1阳性患者的无瘤生存期［（38.43±3.86）个月∶（53.38±2.58）个月］、总生存期［（42.00±3.11）个月∶（53.84±2.19）个月］显著短于ALDH1阴性患者，差异均有统计学意义（χ2=8.490，P=0.004；χ2=11.270，P=0.001）。TGF-β2阳性患者的无瘤生存期［（42.81±3.32）个月∶（54.72±2.50）个月］、总生存期［（44.74±2.68）个月∶（57.18±1.55）个月］显著短于TGF-β2阴性患者，差异均有统计学意义（χ2=4.300，P=0.038；χ2=8.900，P=0.003）。ALDH1与TGF-β2蛋白在乳腺癌组织中的阳性表达呈正相关（r=0.360，P=0.005）。结论 ALDH1可作为判断预后的独立指标，TGF-β2信号通路的激活可能参与调控三阴性乳腺癌中肿瘤干细胞亚群。Objective To investigate the expressions and clinical significances of breast cancer stem cell markers such as aldehyde dehydrogenase 1 （ALDH1） and transforming growth factorβ2 （TGFβ2） in patients with triple negative breast cancer. MethodsSamples of 60 triple negative breast cancer tissues were investigated for the expressions of ALDH1 and TGFβ2 proteins by immunohistochemical staining. The correlation analysis, diseasefree survival analysis and overall survival analysis were performed. ResultsThe positive expressions of ALDH1 protein and TGFβ2 protein in the 60 breast cancer primary lesions were 23 cases （38.33%） and 38 cases （63.33%） respectively. The expression of ALDH1 protein was not correlated with tumor size （χ2=0.307, P=0.580）, histological grade （χ2=4.244, P=0.120）, clinical stage （χ2=0.982, P=0.612） or lymph node metastasis （χ2=1.111, P=0.292）. The expression of TGFβ2 protein was not correlated with histological grade （χ2=4.651, P=0.098）, lymph node metastasis （χ2=3.513, P=0.061）, clinical stage （χ2=1.310, P=0.519） or tumor size （χ2=0.629, P=0.428）. The diseasefree survival time [（38.43±3.86） months vs. （53.38±2.58） months] and the overall survival time [（42.00±3.11） months vs. （53.84±2.19） months] of ALDH1positive patients were significantly shorter than those of ALDH1negative patients, and the differences were statistically significant （χ2=8.490, P=0.004; χ2=11.270, P=0.001）. The diseasefree survival time [（42.81±3.32） months vs. （54.72±2.50） months] and the overall survival time [（44.74±2.68） months vs. （57.18±1.55） months] of TGFβ2 positive patients were significantly shorter than those of TGFβ2negative patients, and the differences were statistically significant （χ2=4.300, P=0.038; χ2=8.900, P=0.003）. The expression of ALDH1 protein was positively correlated with the expression of TGFβ2 protein （r=0.360, P=0.005）. ConclusionThe ALDH1 phen

关 键 词：乳腺肿瘤 肿瘤干细胞 转化生长因子Β2 乙酰脱氢酶1 

分 类 号：R737.9[医药卫生—肿瘤]