乙型肝炎病毒基因型与耐药突变基因的检测分析  

Detection analysis of drug resistant mutations and genotyping of hepatitis B virus

作　　者：储建中[1] 叶光明[1] 

机构地区：[1]武汉大学中南医院医学检验科,武汉430071

出　　处：《临床血液学杂志（输血与检验）》2017年第6期911-913,共3页Journal of Clinical Hematology(Blood Transfusion & Laboratory Medicine)

摘　　要：目的:检测武汉地区乙型肝炎病毒的基因型与耐药突变基因,为临床治疗乙型肝炎患者提供实验室依据。方法:采用PCR-反向斑点杂交法对308例乙肝患者进行乙肝病毒基因型分析,同时检测拉米夫定耐药突变基因和阿德福韦酯耐药突变基因。结果:308例乙肝患者中检出B基因型173例,C基因型78例,B/C混合基因型6例,未检出51例。123例(39.94%)患者的HBV出现拉米夫定耐药,涉及3个位点4个突变型;34例(11.03%)出现阿德福韦酯耐药突变,涉及2个位点2个突变型。结论:武汉地区乙肝病毒存在较高的耐药基因突变率,对乙肝患者进行抗病毒治疗前和治疗过程中,应检测耐药基因是否出现突变。Objective：To investigate the distributions of genotyping and drug resistance mutations of hepatitis B virus in Wuhan.Method：Serum samples were collected from 308 patients with HBV in Wuhan and all the samples were detected by reverse dot blot about the genotyping and drug resistance mutations.Result：Of the 308 patients,173 samples were genotype B and 73 samples were genotype C.Only 6 samples were both genotype B and C.51 samples were undectected.Lamivudine resistant mutations which involving 4 mutations of 3 loci were detected in 123（39.94%）of 308 patients with HBV,while adefovir resistant mutations detected in 34（11.03%）which involving 2 mutations of 2 loci.Conclusion：There was high rate of hepatitis B virus resistance mutations in Wuhan,suggesting that the drug resistance mutations of hepatitis B virus in patients should be detected before and after anti-virus treatment.

关 键 词：乙型肝炎病毒 耐药基因 基因型 突变 

分 类 号：R512.6[医药卫生—内科学]