二氢辣椒碱诱导治疗性低体温对小鼠脑缺血再灌注损伤后的脑保护作用  

Neuroprotective effect of therapeutic hypothermia induced by dihydrocapsaicin on cerebral ischemia reperfusion injury in mice

作　　者：艾玲 乔琼 陈楠 杨涛[1] 唐夏楠 岳静[2] 

机构地区：[1]华中科技大学同济医学院附属同济医院麻醉科,湖北武汉430030 [2]华中科技大学同济医学院附属同济医院生殖医学中心,湖北武汉430030

出　　处：《新乡医学院学报》2017年第12期1058-1062,共5页Journal of Xinxiang Medical University

基　　金：湖北省卫生和计划生育委员会科研面上项目(编号:WJ2015MB005)

摘　　要：目的探讨二氢辣椒碱(DHC)诱导治疗性低体温对小鼠脑缺血再灌注损伤后的脑保护作用及其机制。方法将20只成年野生型(WT)小鼠随机分为WT组和WT+DHC组,每组10只;20只瞬时感受器电位香草酸受体1基因敲除(TRPV1 KO)小鼠随机分为TRPV1 KO组和TRPV1 KO+DHC组,每组10只。所有小鼠建立局灶性脑缺血再灌注损伤模型。WT组和TRPV1 KO组小鼠再灌注开始后皮下泵注生理盐水1.25 mg·kg^(-1)·h^(-1),WT+DHC组和TRPV1 KO+DHC组小鼠再灌注开始后皮下泵注DHC 1.25 mg·kg^(-1)·h^(-1)。所有小鼠再灌注90 min后全部移入22℃的鼠笼中,每隔5 min记录小鼠再灌注期的中心体温变化至再灌注后24 h。在麻醉开始前和再灌注后24 h对小鼠进行神经行为测试评分,测试结束后取小鼠脑组织切片行氯化三苯基四氮唑染色,计算小鼠的脑组织梗死率。结果4组小鼠再灌注0、30、60 min时中心体温比较差异均无统计学意义(P>0.05)。WT组、TRPV1 KO组和TRPV1KO+DHC组小鼠再灌注90 min时的中心体温与0、30、60 min时比较差异均无统计学意义(P>0.05),2、3、4、6、12、24 h时的中心体温与0、30、60、90 min时比较均显著降低(P<0.05);WT组、TRPV1 KO组和TRPV1 KO+DHC组小鼠2、3、4、6、12、24 h时的中心体温在3组同时间点之间两两比较及组内不同时间点之间两两比较差异均无统计学意义(P>0.05)。WT+DHC组小鼠再灌注后90 min及2、3、4、6、12、24 h时的中心体温与0、30、60 min时比较均显著降低(P<0.05),且均显著低于WT组、TRPV1 KO组和TRPV1 KO+DHC组同时间点(P<0.05)。麻醉前4组小鼠神经行为学测试总分比较差异均无统计学意义(P>0.05)。再灌注后24 h 4组小鼠神经行为学测试总分均低于麻醉前(P<0.05),但WT+DHC组小鼠的行为学测试总分明显高于WT组、TRPV1 KO组和TRPV1 KO+DHC组(P<0.05),而WT组、TRPV1 KO组和TRPV1 KO+DHC组小鼠的行为学测试总分比较差异均无统计学意义(P>0.05)。再灌注后24Objective To investigate the neuroprotective effect and its mechanism of therapeutic hypothermia induced by dihydrocapsaicin（ DHC） on cerebral ischemia reperfusion injury in mice. Methods Twenty adult wild type（ WT） mice were randomly divided into WT group and WT ＋ DHC group,ten mice in each group. Twenty transient receptor potential receptor 1（ TRPV1） knockout mice were randomly divided into TRPV1 KO group and TRPV1 KO ＋ DHC group,ten mice in each group. The model of focal cerebral ischemia reperfusion injury was established in all mice. The mice in the WT group and TRPV1 KO group were subcutaneously injected with physiological saline 1. 25 mg·kg^（-1）·h^（-1） after reperfusion. The mice in the WT ＋ DHC group and TRPV1 KO ＋ DHC group were subcutaneously injected with DHC 1. 25 mg·kg^（-1）·h^（-1） after reperfusion. All the mice were moved into the cage at 22 degrees centigrade after 90 minutes of reperfusion. The core body temperature during the reperfusion period was recorded in every 5 minutes to 24 hours after reperfusion. The neurobehavioral score was performed before anesthesia and 24 hours after anesthesia. After the neurobehavioral test,the brain tissue sections of mice were stained with 2,3,5-triphenyltetrazolium chloride; and the infarction rate of the brain tissue was calculated. Results There was no significant difference in the core body temperature among the four groups at 0,30 and 60 minutes after reperfusion（ P 0. 05）. There was no significant difference in the core body temperature between 90 minutes and 0,30 and 60 minutes after reperfusion（ P 0. 05）; but the core body temperature at 2,3,4,6,12 and 24 hours after reperfusion was significantly lower than that at 0,30,60 and 90 minutes after reperfusion in WT group,TRPV1 KO group and TRPV1 KO ＋ DHC group（ P 0. 05）. There was no significant difference in the core body temperature among the WT group,TRPV1 KO group and TRPV1 KO ＋ DHC group at 2,3,4,6,12 and 24 hours after reperfusion（ P 0. 05）.

关 键 词：瞬时感受器电位香草酸受体1 二氢辣椒碱 缺血再灌注损伤 治疗性低体温 脑保护 

分 类 号：R743.3[医药卫生—神经病学与精神病学]