食管鳞状细胞癌组织中PLCE1蛋白表达与患者生存的关系  

Expression of PLCE1 in esophageal squamous cell carcinoma tissues and survival analysis

作　　者：张惠娟[1,2] 赵学科[2] 宋昕[2] 张朋[2] 吕双[2] 王琛[3] 王立东[2] 

机构地区：[1]郑州市第七人民医院体检中心,河南郑州450016 [2]郑州大学第一附属医院河南省食管癌重点开放实验室,河南郑州450052 [3]郑州大学公共卫生学院流行病与卫生统计学教研室,河南郑州450001

出　　处：《新乡医学院学报》2017年第12期1068-1072,共5页Journal of Xinxiang Medical University

基　　金：国家科技部863重点专项资助(编号:SQ2015AA0202183);国家自然科学基金委员会和广东省联合重大项目资助(编号:U1301227);河南省科技重大专项(编号:161100311300);郑州市普通科技攻关项目(编号:20150097)

摘　　要：目的观察食管鳞状细胞癌(ESCC)组织中PLCE1蛋白表达变化,探讨其表达与食管癌患者预后的关系。方法收集河南省食管癌重点开放实验室资料库1997年3月至2011年12月手术切除的85例ESCC患者组织标本,对标本进行PLCE1免疫组织化学染色,采用Kaplan-Meier单因素与Cox多元回归进行生存分析。结果 85例ESCC患者中,失访5例,随访率为94.1%(80/85)。80例ESCC标本中PLCE1阳性表达率为77.5%(62/80)。PLCE1阳性表达与ESCC分化程度、浸润深度、淋巴结转移及家族史无显著相关性(P>0.05)。Kaplan-Meier法生存分析结果显示,PLCE1阳性表达与ESCC患者生存率无显著相关性(P>0.05)。Cox回归分析结果显示,淋巴结转移与ESCC患者的生存时间相关(相对危险度为1.763,95%可信区间:下限为1.008,上限为3.084,P=0.047)。结论 PLCE1蛋白表达参与食管癌变机制,但不能预测ESCC患者预后。Objective To investigate PLCE1 protein expression in esophageal squamous cell carcinoma（ ESCC） tissues,and understand the effect of PLCE1 protein expression on the prognosis of patients with ESCC. Methods The PLCE1 protein were detected in 85 patients with surgically resected ESCC paraffin-embedded tissue using immunohistochemical staining,the patients＇ information were selected from March 1997 to December 2011 in the database of the Henan Key Laboratory for Esophageal Cancer Research,and the survival analysis were operated with Kaplan Meier single factor and Cox multivariate regression. Results In the 85 cases,the lost to follow-up were 5 cases,the follow-up rate was 94. 1%（ 80/85）. In the 80 cases with ESCC,the positive expression rate of PLCE1 was 77. 5%（ 62/80）. PLCE1 positive expression was not significantly associated with differentiation,infiltration depth,lymph node metastasis and family history with ESCC（ P 0. 05）. The univariate survival analysis by Kaplan-Meier showed that protein expression of PLCE1 had no significant association with overall survival（ P 0. 05）. Cox regression survival analysis showed that the lymph node metastasis was associated with the survival time of ESCC patients[the relative risk were 1. 763,95% CI（ 1. 008,3. 084）,P = 0. 047]. Conclusion The expression of PLCE1 protein was involved in mechanism of ESCC,and might not predicted the prognosis of the patients with ESCC.

关 键 词：食管鳞状细胞癌 PLCE1 生存分析 

分 类 号：R735.1[医药卫生—肿瘤]