血清肿瘤坏死因子-α和胶质纤维酸性蛋白水平与脑胶质瘤恶性程度及患者预后的相关性  

Correlation between the levels of serum tumor necrosis factor-α,glial fibrillary acidic protein and the malignant degree and prognosis of patients with brain glioma

作　　者：刘晓斌[1] 李民[1] 黄卫东[1] 

机构地区：[1]陕西省人民医院神经外科,陕西西安710068

出　　处：《新乡医学院学报》2017年第12期1097-1100,共4页Journal of Xinxiang Medical University

基　　金：陕西省自然科学基础研究计划项目(编号:S2016YFJW1635)

摘　　要：目的探讨血清肿瘤坏死因子-α(TNF-α)和胶质纤维酸性蛋白(GFAP)水平与脑胶质瘤恶性程度及患者预后的相关性。方法选取2013年3月至2015年9月陕西省人民医院收治的脑胶质瘤患者90例,依据恶性程度病理学分级将患者分为低度恶性组43例和高度恶性组47例;选择同期体检健康志愿者52例作为对照组。脑胶质瘤患者分别于治疗前、术后第14天、放射治疗和化学治疗后第1天采集晨起空腹静脉血5 mL,对照组受试者于体检当日采集晨起空腹静脉血5 mL,采用酶联免疫吸附法测定血清TNF-α水平,双抗体夹心法测定血清GFAP水平。结果治疗前,低度恶性组和高度恶性组患者血清TNF-α、GFAP水平显著高于对照组(P<0.05),高度恶性组患者血清TNF-α、GFAP水平显著低于低度恶性组(P<0.05)。低度恶性组患者术后第14天及放射治疗和化学治疗后血清TNF-α、GFAP水平显著低于治疗前(P<0.05),放射治疗和化学治疗后血清TNF-α、GFAP水平显著低于术后第14天(P<0.05)。高度恶性组患者术后第14天血清TNF-α水平显著低于治疗前(P<0.05),但GFAP水平与治疗前比较差异无统计学意义(P>0.05);高度恶性组患者放射治疗和化学治疗后血清TNF-α、GFAP水平显著低于治疗前和术后第14天(P<0.05)。治疗后,完全缓解患者血清TNF-α及GFAP水平显著低于部分缓解、疾病稳定和疾病进展患者(P<0.05),部分缓解患者血清TNF-α及GFAP水平显著低于疾病稳定和疾病进展患者(P<0.05),疾病稳定患者血清TNF-α及GFAP水平显著低于疾病进展患者(P<0.05)。结论脑胶质瘤患者血清TNF-α和GFAP水平与肿瘤恶性程度、治疗效果有一定的相关性,可以作为临床评估脑胶质瘤恶性程度、治疗效果及预后的生物学标志物。Objective To investigate the correlation between the levels of serum tumor necrosis factor-α（ TNF-α）,glial fibrillary acidic protein（ GFAP） and the malignant degree and prognosis of patients with brain glioma. Methods A total of 90 patients with brain glioma were collected from March 2013 to September 2015 in Shaanxi Provincial People＇s Hospital. The patients were divided into low malignant degree group（ n = 43） and high malignant degree group（ n = 47） according to the pathological grade of malignant degree. Fifty-two healthy volunteers were selected as control group at the same period. The fasting venous blood 5 mL of the patients with brain glioma was collected at the time points of pretherapy,the 14 th day after operation,the first day after radiotherapy and chemotherapy. The fasting venous blood 5 mL was collected on the day of physical examination in the control group. The level of serum TNF-α was detected by enzyme-linked immunosorbent assay,and the level of serum GFAP was measured by double antibody sandwich method. Results The levels of serum TNF-α and GFAP in the low malignant degree group and high malignant degree group were significantly higher than those in the control group（ P 0. 05）,and the levels of serum TNF-α and GFAP in the high malignant degree group were significantly lower than those in the low malignant degree group before treatment（ P 0. 05）. The levels of serum TNF-α and GFAP at the time points on the 14 th day after operation and after radiotherapy and chemotherapy were significantly lower than those before treatment（ P 0. 05）; the levels of serum TNF-α and GFAP after radiotherapy and chemotherapy were significantly lower than those on the 14 th day after operation in the low malignant degree group（ P 0. 05）. The level of serum TNF-α on the 14 th day after operation was significantly lower than that before treatment in the high malignant degree group（ P 0. 05）. There was no significant difference in serum GFAP level between the 14 th day after

关 键 词：脑胶质瘤 肿瘤坏死因子-α 胶质纤维酸性蛋白 预后 

分 类 号：R739.41[医药卫生—肿瘤]