TATp与SDF-1修饰的载多西紫杉醇靶向超声造影剂体内成像及初步治疗实验  

In vivo imaging and preliminary treatment experiments of docetaxel-loaded ultrasound microbubbles decorated with TATp and SDF-1 agents

作　　者：易品诗 邱丽华[2] 何力 高志[4] 赵欣[5] 李攀[6] 李颖[2] 

机构地区：[1]口腔疾病与生物医学重庆市重点实验室,重庆401120 [2]重庆医科大学附属口腔医院颌面外科,重庆401120 [3]重庆市高校市级口腔生物医学工程重点实验,重庆401120 [4]重庆医科大学附属第二医院口腔科,重庆400010 [5]重庆第二师范学院重庆市功能性食品协同创新中心,重庆400067 [6]重庆医科大学超声影像学研究所,重庆400010

出　　处：《重庆医科大学学报》2017年第12期1592-1597,共6页Journal of Chongqing Medical University

基　　金：重庆市科技计划资助项目(编号:cstc2016shmszx00010)

摘　　要：目的:探索由穿膜肽(transcriptional activator protein,TATp)和基质细胞衍生因子-1(stromal cell-derived factor-1,SDF-1)修饰的载多西紫杉醇超声造影剂对舌癌颈部淋巴组织转移灶的治疗效果。方法:以兔VX2舌癌颈淋巴转移模型、SCC-15细胞、超声造影剂为研究对象,将兔模型分为A、B、C、D组,分别用空白微泡(A)、靶向载药微泡(B)、单纯药物(C)和空白(D)处理;将细胞分为A、B、C、D、E、F组,分别用空白(A)、单纯药物组(B)、载药微泡(C)、靶向载药微泡(D)、靶向载药微泡+超声(E)和超声(F)处理细胞,通过测量淋巴结体积、免疫组化和细胞凋亡检验实验结果。结果:超声检查见颈部转移性淋巴结,实验中各组的肿瘤抑制率分别为5.3%、25.4%、22.3%、0.0%,组间比较:PAD=0.436,PBD=0.010,PCD=0.008,PBC=0.723;各组p53阳性率分别为(29.8±3.3)%,(3.8±2.8)%,(3.8±3.1)%,(30.8±3.6)%,组间比较:P_(AD)=0.663,P_(BD)=0.000,P_(CD)=0.000,P_(BC)=1.000。细胞实验中各组细胞凋亡率分别为(17.82±1.39)%,(57.61±2.37)%,(31.87±2.18)%,(46.28±1.65)%,(67.34±2.22)%,(14.17±8.13)%,组间比较P_(AB)=0.000,P_(AC)=0.001,P_(AD)=0.000,P_(AE)=0.000,P_(AF)=0.486,P_(EB)=0.007,P_(EC)=0.000,P_(ED)=0.000,P_(EF)=0.000。结论:TATp和SDF-1修饰的载药超声微泡在治疗中有一定作用,能为舌癌颈淋巴结转移灶的临床治疗提供参考。Objective：To explore the imaging effect and treatment effect of the ultrasonic microbubble modified by transcriptional activator protein（TATp）and stromal cell-derived factor-1（SDF-1）carrying docetaxel lipid in metastatic lymph node lymph node of tongue cancer.Methods：A control study was made with the VX2 cervical lymph node metastasis in rabbits model,SCC-15 cell and the ultrasonic microbubble modified by TATp and SDF-1 carrying docetaxel.The rabbit model was divided into A,B,C,D groups,respectively dealing with blank microbubbles（A）,targeted microbubbles（B）,drug（C）and blank（D）.The cells were divided into：A,B,C,D,E,F groups,respectively dealing with blank（A）,drug group（B）and microbubbles（C）,targeted microbubbles（D）,targeted microbubbles ＋ ultrasound（E）and ultrasound（F）.The results were tested by measuring lymph node＇s volume,immunohistochemistry,and apoptosis.Results：The ultrasonography revealed metastatic cervical lymph nodes.The tumor inhibitory rates in each group were 5.3%,25.4%,22.3%,0.0%.Comparison between groups showed that：PAD=0.436,PBD=0.010,PCD=0.008,PBC=0.723.The positive rates of p53 were（29.8±3.3）%,（3.8±2.8）%,（3.8±3.1）%,（30.8±3.6）%.Comparison between groups were PAD=0.663,PBD=0.000,PCD=0.000,PBC=1.000.The apoptotic rates were（17.82±1.39）%,（57.61±2.37）%,（31.87 ±2.18）%,（46.28 ±1.65）%,（67.34±2.22）%,（14.17±8.13）%.Comparisons between groups were PAB=0.000,PAC=0.001,PAD=0.000,PAE=0.000,PAF=0.486,PEB=0.007,PEC=0.000,PED=0.000,P_（EF）=0.000.Conclusion：Ultrasounic microbubble modified by TATp and SDF-1 play a certain role in the treatment,and can provide reference for the clinical treatment of cervical lymph node metastasis.

关 键 词：超声造影剂 靶向治疗 舌癌 体内实验 多西紫杉醇 

分 类 号：R-332[医药卫生]