乙醇-硫酸铵双水相提取鱼腥草总黄酮  

Extraction of Flavonoids from Herba houttuyniae Using Ethanol-Ammonium Sulfate Aqueous Two Phase System

作　　者：郝红英[1] 周芳[1] 王改利[1] 高阳逸 丁小娟[1] 

机构地区：[1]黄河科技学院医学院,郑州450063

出　　处：《湖北农业科学》2017年第24期4844-4846,共3页Hubei Agricultural Sciences

基　　金：河南省高等学校重点科研项目(15B350003);黄河科技学院大学生实践创新项目(2015XSCXCY002)

摘　　要：通过单因素试验优化了影响乙醇-硫酸铵双水相提取鱼腥草(Herba houttuyniae)总黄酮提取率的因素,进一步通过正交试验确定了各因素的影响程度,顺序为乙醇浓度>提取温度>提取时间>料液比。优化后的提取条件为脱脂鱼腥草粉末2.0 g,乙醇用量32.50%(w)、硫酸铵16.00%(w)、料液比1∶15、提取温度60℃、提取时间30 min,在该条件下,鱼腥草总黄酮提取率为3.16%。The factors affecting the extraction yield of flavonoids were optimized by single factor test and orthogonal design to investigate the effect on the extraction technology of flavonoids from herba houttu-yniae using ethanol-ammonium sulfate aque- ous two phase system. The influence degree of each factor was determined by orthogonal experiment： ethanol content〉extraction temperature〉extraction time〉solid-liquid ratio. The optimal extraction technology was that skim herba houttuyniae was 2.0 g, ethanol 32.50% （w）, ammonium sulfate 16.00% （w）, solid-liquid ratio 1：15, temperature 60℃, and time was 30 min. Under this condition, the extraction rate of flavonoids was 3.16%.

关 键 词：鱼腥草(Herba houttuyniae) 双水相 总黄酮 提取工艺 

分 类 号：R284.2[医药卫生—中药学]