金樱子多糖单糖组成的TMS柱前衍生化/GC-MS研究  

GC-MS Analysis of Monosaccharide Composition of TMS Pre-column Derivatization of Rosa laevigata

作　　者：陈传平 吴剑锋[1,2,3] 陈乃东 

机构地区：[1]皖西学院生物与制药工程学院,六安237012 [2]皖西中药与天然药物工程技术研究中心,六安237012 [3]皖西卫生职业学院,六安237005

出　　处：《天然产物研究与开发》2017年第12期2063-2067,共5页Natural Product Research and Development

基　　金：安徽省教育厅自然科学研究重点项目(KJ2015A361);国家自然科学基金(81573536;81274021);中国博士基金特别资助(第后9批)(2016T90559);中国博士后研究面上项目(2014M551791);安徽省自然基金面上项目(1608085MH221)

摘　　要：探讨金樱子多糖的含量及其单糖组成,为金樱子质量评价及临床用药提供参考依据。本文采用水提-醇沉法获得金樱子多糖,以葡萄糖为标准品,蒽酮-硫酸比色法测定多糖含量;多糖酸解后经三甲基硅烷(TMS)衍生化,以标准品单糖为对照,采用气-质联用法(GC-MS)测定多糖的单糖组成。结果表明,本实验条件下,测得金樱子多糖提取得率为29.38%;金樱子多糖中单糖由阿拉伯糖、鼠李糖、木糖、甘露糖、半乳糖、葡萄糖、果糖组成,其中葡萄糖、甘露糖、果糖和半乳糖在金樱子多糖中占比较大,分别为65.03%、7.36%、16.11%及8.87%。To explore the content and the monosaccharide composition of the polysaccharide in Rosa laevigata Mickx and provide references for the quality control and the pharmaceutical utilization of R. laevigata, the total polysaccharide was obtained by water extraction and alcohol precipitation method. The polysaccharide content was detected by anthrone sul- furic acid colorimetry using the glucose calibration. Then the monosaccharide compositions of the polysaccharide were an- alyzed by TMS pre-column GC-MS method. The results showed that the yield of total polysaccharide was 29.38%. The polysaccharide was composed by arabinose, rhamnose, xylose, mannose, galactose, glucose, galacturonic acid and fruc- tose, and the contents of glucose, mannose, fructose and galactose were reached to 65.03% , 7.36%, 16. 11% and 8. 87% ,respectively, obviously higher than the other 4 monosaccharides in R. laevigata polysaccharide.

关 键 词：金樱子 多糖 单糖 气相色谱-质谱联用 

分 类 号：R283.3[医药卫生—中药学]