红冬蛇菰提取物抗氧化及抑制亚硝化作用研究  

Antioxidant and Inhibitory Effect on Nitrosation of Extracts from Balanophora harlandii

作　　者：张声源[1] 庄远杯 罗铝铿[1] 陈一村[2] 聂华[1] 

机构地区：[1]嘉应学院医学院,梅州514031 [2]汕头大学医学院,汕头515041

出　　处：《天然产物研究与开发》2017年第12期2081-2086,共6页Natural Product Research and Development

基　　金：广东省科技计划(2014A020221061;2015A090905019);广东省医学科研基金(A2015354);梅州市科技研究与开发资金(201408);广东省大学生创新创业项目(201510582060)

摘　　要：对红冬蛇菰不同溶剂提取物的抗氧化活性及抑制亚硝化作用进行比较研究。采用DPPH法、ABTS法和普鲁士蓝法分别测定各提取物的自由基清除能力和铁离子还原力,以评价红冬蛇菰的抗氧化能力;采用盐酸萘乙二胺法和α-萘胺法分别测定红冬蛇菰各提取物对亚硝酸盐的清除率和对亚硝胺合成的阻断率,以评价红冬蛇菰的抑制亚硝化作用。实验结果表明,红冬蛇菰各提取物中乙酸乙酯提取物清除DPPH自由基(IC_(50)=0.06±0.001 mg/mL)和ABTS自由基(IC_(50)=0.07±0.001 mg/mL)能力以及对铁离子还原能力最强,并显著强于其他提取物(P<0.01),其中清除ABTS自由基的能力显著高于Vc(IC_(50)=0.10±0.002 mg/mL)(P<0.01);在模拟人体胃液的条件下(pH 3.0,温度37℃),各提取物能有效地清除亚硝酸盐以及阻断亚硝胺合成,并随着反应质量浓度增加清除率和阻断率增大最后趋于平缓,其中乙酸乙酯提取物对亚硝酸盐的清除能力(IC_(50)=1.91±0.022 mg/mL)和亚硝胺合成的阻断能力(IC_(50)=8.44±0.091 mg/mL)均显著强于其他提取物(P<0.01)。红冬蛇菰提取物属天然产物,具有较好的抗氧化活性和抑制亚硝化作用,因此在抗氧化剂的研究方面具有潜在的开发利用价值,同时极有可能是一种新的防癌抗癌天然保健品。The antioxidant activities and inhibition on nitrosation of different solvent extracts from Balanophora harlandii were researched by comparative study. Antioxidant activities of B. harlandii extracts were evaluated by methods of DPPH radical scavenging, ABTS radical scavenging and Prussian blue. Nitrate removal rate and blocking rate of nitrosamine of different solvent extracts were determined with the methods of hydrochloric acid naphthalene ethylene diamine and et- naphthylamine to evaluate its inhibitory effect on nitrosation reactions, respectively. The results showed that the ethyl ace- tate extract of B. harlandii had the strongest free radical scavenging and ferric icon reducing power （ P 〈 0.01 ）, the ABTS free radical scavenging ability of which was higher than Vc （P 〈 0.01 ）. In simulated conditions of human gastric juice （pH 3.0, temperature 37℃ ）, B. harlandii extracts can effectively scavenge nitrite and block the nitrosamines syn- thesis. With the concentration increasing, the rates of scavenging and block trended gently. However, the activity of scav- enge nitrite and block the nitrosamines synthesis of B. harlandii ethyl acetate extract （ IC50=1.91 ± 0. 022,8.44 ±0. 091 mg/mL,respectively） were the higher than that of other extracts of B. harlandii. The extracts of B. harlandii had strong antioxidant activity and nitrosation inhibition effect, which had potential value for development and utilization in the antioxidant research and can be most potentially developed as a new anti-cancer natural health product.

关 键 词：红冬蛇菰 抗氧化 抑制亚硝化 自由基 DPPH 

分 类 号：R932[医药卫生—生药学]