RNAi技术沉默大鼠骨髓间充质干细胞OPG基因对RANKL/OPG表达变化研究  

Effect of RNAi Silencing OPG Gene on RANKL/OPG Expression in Rat Bone Marrow Mesenchymal Stem Cells

作　　者：韦颂观 陈慧鸿 庞博 谢柳蓉 吴修团 李文良 廖红兵[1] 

机构地区：[1]广西医科大学附属口腔医学院口腔修复科,广西南宁530021

出　　处：《口腔医学研究》2017年第12期1254-1257,共4页Journal of Oral Science Research

基　　金：国家自然基金资助项目(编号:81560190);广西自然科学基金(编号:2016GXNSFAA380289)

摘　　要：目的:探讨RNAi技术体外沉默大鼠骨髓间充质干细胞(BMSCs)OPG基因提高RANKL/OPG比例的可行性。方法:设立空载组(空慢病毒载体转染BMSCs)和OPG沉默组(含OPG基因干扰片段的慢病毒载体转染BMSCs),RT-PCR检测OPG及RANKL的mRNA表达变化,Western blot检测OPG及RANKL的蛋白表达变化。结果:与空载组比较,OPG沉默组OPG的mRNA和蛋白表达显著降低,RANKL的mRNA表达显著升高,差异均有统计学意义(P<0.05);两组RANKL的蛋白表达差异无统计学意义(P>0.05);OPG沉默组RANKL/OPG mRNA和蛋白表达比例均显著升高,差异均有统计学意义(P<0.05)。结论:以慢病毒为载体,通过RNAi技术体外沉默大鼠骨髓间质干细胞OPG基因提高RANKL/OPG比例的方法是可行的。Objective： To explore the feasibility of increasing the RANKL/OPG ratio by using the RNAi technique to silence rat OPG gene of bone marrow mesenchymal stem cells （BMSCs） in vitro. Methods： BMSCs were set up in the empty vector group and OPG silencing group. RT PCR was used to detect the mRNA expression of OPG and RANKL. Western blot was used to detect the protein expression of OPG and RANKL. Results： The expressions of OPG mRNA and protein in OPG silencing group were significantly inhibited and the expression of RANKL mRNA was significantly increased （P〈0.05） compared with the empty vector group. There was no significant difference in the expressions of RANKL protein between two groups （P〉0.05）. The expressions of RANKI./OPG mRNA and protein in OPG silencing group were significantly increased. The difference was statistically significant （P〈0.05）. Conclusion： It is feasible to increase the RANKL/OPG ratio by using the lentivirus as vector and silencing OPG gene of rat BMSCs by RNAi technique in vitro.

关 键 词：骨髓间充质干细胞OPG RNAI RANKL RANKL/OPG 

分 类 号：R781[医药卫生—口腔医学]