介孔硅载七叶皂苷钠对粪肠球菌抑菌作用的研究  

Study on Bacteriostasis of Sodium Aescinate-loaded Nanomesoporous Silica on Enterococcus Faecalis

作　　者：黄大维 李奉华[2] 雷焯 姜海敏 

机构地区：[1]厦门市妇幼保健院口腔科,福建厦门361003 [2]中南大学湘雅口腔医学院,湖南长沙410008 [3]厦门大学附属中山医院口腔科,福建厦门361003

出　　处：《口腔医学研究》2017年第12期1286-1290,共5页Journal of Oral Science Research

摘　　要：目的:探讨氨基改性前后的介孔硅载七叶皂苷钠(SA-SBA-15及SA-NH_2-SBA-15)和氢氧化钙Ca(OH)_2对粪肠球菌的抑菌作用。方法:液体稀释法测SA-NH_2-SBA-15、SA-SBA-15和Ca(OH)_2的最低抑菌浓度(MIC);液体扩散法观察SA-NH_2-SBA-15、SA-SBA-15和Ca(OH)_2对粪肠球菌抑菌圈的大小;SA-NH_2-SBA-15、SA-SBA-15和Ca(OH)_2与菌液共培养168h,每隔24h取0.1mL液体另作厌氧培养24h,评价抑菌效果。结果:SA-NH_2-SBA-15、SA-SBA-15的MIC为3.5g/L,Ca(OH)_2的MIC为4.0g/L。SA-NH_2-SBA-15、SA-SBA-15和Ca(OH)_2浓度大于MIC时,抑菌圈大小与浓度呈依赖性增高,差异具有统计学意义(P<0.05)。72h后至168h,SA-SBA-15和SA-NH_2-SBA的菌落数明显少于Ca(OH)_2。96h后,SA-NH_2-SBA-15组的菌落数明显少于SA-SBA-15组,差异具有统计学意义(P<0.05)。结论:SA-NH_2-SBA-15、SA-SBA-15及Ca(OH)_2对粪肠球菌具有一定的抗菌作用;SA-NH_2-SBA-15、SA-SBA-15的抑菌效果优于Ca(OH)_2;SA-NH_2-SBA-15对粪肠球菌的药效长于SA-SBA-15,长于Ca(OH)_2。Objective： To investigate the bacteriostasis of sodium aescinate--loaded nanomesoporous silica before and after animo-- modified （SA-- SBA-- 15 and SA-- N H2 -- SBA-- 15 ） and calcium hydroxide （Ca （ O H） 2 ） on En terococcus faecalis. Methods： The MIC of SA--NH2--SBA--15, SA-SNA--15, and Ca（OH）2 on Enterococcus faecalis were determined by liquid dilution susceptibility experiment. The diameter of inhibition zone on Enterococ- cus faecalis was observed and measured with the method of liquid diffusion. To evaluate the antibacterial effect, SA --NH2 --SBA--15, SA SBA--15, and Ca（OH）2 were cultured with bacterial solution for 168 h,and then 0.1 mL solution was removed for anaerobic cultivation at each 24h. Results： The liquid dilution test showed： the MIC of SA --NH2--SBA--15 and SA--SBA--15 was 3.5mg/mL, the MIC of Ca（OH）2 was 4.0mg/mL. When the concen- trations of SA--NH2--SBA--15, $A--SBA--15 and Ca（OH）2 was higher than the MIC, the inhibition zone size increased along with the concentrations （P〈0.05）. From 72 h to 168 h, the CFU of SA--NH2 SBA--15 and SA --SBA--15 was less than that of Ca（OH）2. After 96 h, the CFU of SA NH2--SBA--15 was obviously less than that of SA--SBA--15 （P〈0.05）. Conclusion： Three kinds of drugs had a certain inhibitory effect on Enterococcus faecalis. The bacteriostatic effect of SA--NH2 --SBA--15 and SA--SBA--15 was better than that of Ca（OH）2. The efficacy of SA-- NH2 -- SBA-- 15 on Enterococeus faecalis was longer than that of SA-- SBA-- 15 and Ca （OH）2.

关 键 词：介孔硅 粪肠球菌 七叶皂苷钠 氢氧化钙 

分 类 号：R781.05[医药卫生—口腔医学]