OLP患者外周血T细胞B7-H1及PD-1的表达情况及与疾病状态的关系研究  

Expression of B7-H1 and PD-1 on Peripheral Blood T Cells in OLP Patients and Its Correlation with Disease

作　　者：任莉[1] 韩杰 徐明明[2] 王隽[1] 

机构地区：[1]北京华信医院(清华大学第一附属医院)口腔科,北京100016 [2]北京大学口腔医院特诊科,北京100101

出　　处：《口腔医学研究》2017年第12期1319-1322,共4页Journal of Oral Science Research

摘　　要：目的:通过检测口腔扁平苔藓(oral lichen planus,OLP)患者外周血T细胞B7-H1及PD-1的表达情况及与疾病状态的关系,以此来探讨B7-H1/PD-1信号通道在OLP患者T细胞免疫中的作用。方法:选择22例OLP患者,12例健康成人作为对照。应用REU计分系统及视觉模拟评分法(VAS)对OLP患者的疾病状态进行计分。密度梯度离心法分离OLP组和对照组外周血中的单个核细胞,流式细胞术检测单个核细胞中T细胞表面B7-H1及PD-1的表达水平。贴壁法去除单核细胞后,将OLP患者的淋巴细胞与健康成人淋巴细胞混合培养,加入抗B7-H1单克隆抗体阻断B7-H1/PD-1信号通道,培养5d后应用XTT方法检测细胞增殖情况。结果:CD3+B7-H1+、CD3+PD-1+的表达百分率在OLP和正常对照组之间存在有明显差异(P<0.05)。CD3+B7-H1+在OLP组的表达水平与疾病状态计分正相关(P=0.002),而CD3+PD-1+的表达水平与疾病的临床状态计分无关(P=0.537)。在混合淋巴细胞培养中,使用抗B7-H1单克隆抗体阻断B7-H1/PD-1信号通道后,淋巴细胞增殖情况与对照组无明显差异(P>0.05)。结论:OLP患者外周血T细胞中的CD3+B7-H1+、CD3+PD-1+很可能参与了OLP的发病;CD3+B7-H1+对OLP的疾病进展可能产生一定作用。Objective： To compare the expression of B7-H1 and PD-1 on peripheral blood T cells in oral lichen planus （OLP） patients and controls, and to explore its relationship with the disease. Methods： Twenty-two pa- tients of OLP and twelve healthy adults were chosen. The scores of OLP disease activity were assessed according to the REU scoring system and the visual analogue scale （VAS） as described in the previous studies. Peripheral blood mononuclear cells （PBMCs） were isolated by density gradient centrifugation. The expression of BT--H1 and PD--1 on T cells in PBMCs were tested by two--color flow cytometry. After monocyte cells were removed by adhesion, the lymphocyte cells of OLP patients and controls were mixed for culture using anti--BT--H1 mAbs to block the B7 --H1/PD--1 pathway, then the proliferation of lymphocyte cells were detected through XTT method after 5 days. Results：Significant differences were found among OLP patients and controls in the expression of B7--H1 and PD- 1 on T cells （P 〈 0.05）. The expression of B7- H1 on T cells in OLP positively associated with the disease activity score （P=0. 002）. However, there had no relationship between the expression of PD--1 on T cells and the disease activity score in OLP （P=O. 537）. After blocking the B7-H1/PD--1 pathway with anti-B7-H1 mAbs, the pro- liferation of T cells was not significant different between the control group and the OLP group after mixed lympho- cyte cell cultures （P〉0.05）. Conclusion; The results indicated that B7--H1 and PD--1 might play importantroles in the pathogenesis of OLP. B7--H1 in T cells might relate with the progression of OLP disease.

关 键 词：口腔扁平苔藓 B7-H1/PD-1信号通道 T细胞 混合淋巴细胞培养 

分 类 号：R781.5[医药卫生—口腔医学]