炎症相关性结直肠癌小鼠模型建立及血管生成抑制因子1在结直肠癌中的表达  

Establishment of mice model of inflammation-associated colorectal cancer and expression of vasohibin-1 in colorectal cancer

作　　者：王磊[1] 时军利[1] 李炳庆[1] 

机构地区：[1]承德医学院附属医院消化内科,河北承德067000

出　　处：《中国医药导报》2017年第36期17-20,F0003,共5页China Medical Herald

基　　金：河北省卫生计生委医学科学研究重点课题计划(20150021)

摘　　要：目的探讨炎症相关性结直肠癌小鼠模型的建立,及血管生成抑制因子1(VASH-1)在结直肠癌中的表达。方法将雄性小鼠(C57BL/6)30只按体重大小随机分为两组。A组给予氧化偶氮甲烷(AOM)10 mg/kg右侧腹腔注射,后于第1、4、7周给予1.5%~2.5%葡聚糖硫酸钠(DSS)自由饮用7 d,其余时间自由饮清水。B组给予生理盐水0.1 m L/10 g右侧腹腔注射,此后给予清水自由饮用。均在第10周末处死小鼠,查看成瘤情况。免疫组织化学染色法探讨VASH-1在结直肠癌组织中的表达。结果造模结束后A组成瘤率为53.33%,病理提示为腺癌,B组成瘤率为0.00%,两组成瘤率比较差异有统计学意义(P<0.05)。VASH-1在结直肠癌中高表达,在正常结直肠组织中低表达,差异有统计学意义(P<0.05)。结论本方法建立的炎症相关性结直肠癌小鼠模型成瘤率较高。VASH-1在结直肠癌组织中的表达高于正常结直肠组织。Objective To investigate the establishment of mice model of inflammation-associated colorectal cancer and expression of vasohibin-1 （VASH-1） in colorectal cancer. Methods Thirty male mice （C57BL/6） were randomly divided into two groups according to the body weight. Group A was given right intraperitoneal administration of azoxymethane （AOM） 10 mg/kg first, and free drink of 1.5%-2.5% dextran sulfate sodium （DSS） at the first, fourth, seventh week later for 7 d, which drunk distilled water on the other time. Group B was given right intraperitoneal administration of normal saline 0.1 mL/10 g, then the mice were given distilled water on the other time. The mice of both groups were killed at the end of tenth week to observe the conditions of tumor formation. The immunohistochemical staining was performed to evaluate the expression of VASH-1 in the of tissue colorectal cancer. Results After modeling, the tumor formation rate of group A was 53.33%, with the pathologic findings of adenocarcinoma, which of group B was 0.00%, the difference was statistically significant （P 〈 0.05）. VASH-1 had high expression in the colorectal cancer and low expression in normal colorectal tissues, with significant difference （P 〈 0.05）. Conclusion The tumor formation rate of mice model of inflammation-associated colorectal cancer induced by this method is high. The expression of VASH-1 in colorectal cancer is higher than that in normal colorectal tissues.

关 键 词：炎症性肠病 结直肠肿瘤 动物模型 血管生成抑制因子 

分 类 号：R735.3[医药卫生—肿瘤]