Study on Callus Induction from Immature Embryos and Plant Regeneration of Different Genotypes of Maize  

作　　者：Hao DONG Jing CHEN 

机构地区：[1]Tai'an Academy of Agricultural Sciences, Tai' an 271000, China [2]Daiyue Agricultural Bureau, Tai' an 271000, China

出　　处：《Agricultural Biotechnology》2017年第6期29-31,共3页农业生物技术（英文版）

基　　金：Supported by Science and Technology Development Plan of Shandong Province;National Key Laboratory Open Project of Crop Biology(2014KF11)

摘　　要：The induction, subculture and differentiation of callus from immature embryos of maize （ Zea mays L. ） inbred line were studied and optimized. The re- suits revealed that, 2 mg/L 2,4-D was the optimal concentration to induce embryonic callus. Calli induced from inbred Qi319 and LY92 had good morphology and high regeneration. The embryos of the two inbred lines were selected as explants to establish efficient and stable tissue culture and transformation system.The induction, subculture and differentiation of callus from immature embryos of maize （ Zea mays L. ） inbred line were studied and optimized. The re- suits revealed that, 2 mg/L 2,4-D was the optimal concentration to induce embryonic callus. Calli induced from inbred Qi319 and LY92 had good morphology and high regeneration. The embryos of the two inbred lines were selected as explants to establish efficient and stable tissue culture and transformation system.

关 键 词：Maize （Zea mays L. ） Immature embryos CALLUS SUBCULTURE DIFFERENTIATION 

分 类 号：S513[农业科学—作物学]