红颜草莓茎尖组织培养的器官发生途径研究  

Organogenesis Approaches in Tissue Culture of Stem Tips of Benihoppe Strawberry(Fragaria ananasosa Duch.)

作　　者：王晓玲 唐丽萍 范雨 罗晓波[3] 曾德刚 

机构地区：[1]凉山州劳动职业技术学校,四川西昌615000 [2]四川师范大学生命科学学院,四川成都610101 [3]四川省自然资源科学研究院,四川成都610015 [4]凉山州农业学校,四川西昌615000

出　　处：《四川林业科技》2017年第6期40-44,共5页Journal of Sichuan Forestry Science and Technology

基　　金：凉山州技术研究与推广应用项目(15YYJS0051)优质草莓品种引进及组培脱毒育苗技术研究

摘　　要：为探究红颜草莓茎尖组培苗器官再生途径,以其茎尖为外植体进行组织培养,观察其器官再生过程中的形态学和解剖学变化特点。结果表明:红颜草莓茎尖离体再生的芽原基起源于茎尖形态学上端表皮层的薄壁细胞,属外起源;表皮层的薄壁细胞先分化形成芽原基,随后芽原基周围的皮层薄壁细胞再分化出叶原基;芽原基和叶原基共同发育形成不定芽;不定芽再生途径为器官型。不定芽基部组织受激素诱导于形成层分化出根原基,同时不定芽基部表皮层细胞也可分化出根原基。故不定根外起源方式既有内起源也有外起源,但不定根都属于诱生根原基。不定芽和不定根中的输导组织将整个组培苗连接成一个整体,形成完整植株。To study the organogenesis approaches in tissue culture of stem tips of Benihoppe strawberry （ Fragaria ananassa Duch. ）, stem tips were used as explants for tissue culture, aiming to observe the morphological and anatomical changes during organic regeneration. Results showed that the regenerative bud primodium in vitro of Benihoppe Strawberry （F. ananassa Duch. ） derived from the parenehyma cells in epidermis on the morphologic apex of stem tips, so it originated in exogenous way;Parenchyma cells in epidermis differentiated to form bud primordium, then the leaf primordium would be differentiated from parenehyma cells in epidermis near it ; the bud primordium and leaf primordium grew together to form adventitious shoots;Organogenesis type was the way for the bud primordium to regenerate. The tissue of adventitious shoots base was induced by hormone , which would cause the differentiation of root primordi- um in cambium , the epidermic cells of adventitious shoots base could form root primordium too. There- fore , the way that adventitious roots originated could be exogenous or endogenous, but the adventitious roots were all the induced root primordia. At last, the conducting tissue in adventitious shoots and roots connected the whole tissue culture seedling into a complete plant.

关 键 词：红颜 组织培养 器官发生途径 解剖学 

分 类 号：S668.4[农业科学—果树学]