液相色谱串联质谱法测定白菜中的甲醛含量  

Determination of formaldehyde in Chinese cabbage by high performance liquid chromatography tandem mass spectrometry

作　　者：顾亚萍 乔方[2] 方长发[2] 胡祥娜 王瑞 林慧纯 张士瑞 禹绍周 张晓强 

机构地区：[1]深圳市农产品质量安全检验检测中心,广东深圳518040 [2]深圳职业技术学院应用化学与生物技术学院,广东深圳518055

出　　处：《食品与发酵工业》2017年第12期214-217,共4页Food and Fermentation Industries

基　　金：现代农业产业技术体系建设专项资金项目(CARS-33-20)

摘　　要：文中使用2,4-二硝基苯肼衍生法使白菜中甲醛生成甲醛-2,4-二硝基苯腙,再使用乙腈提取富集甲醛-2,4-二硝基苯腙(衍生物),后经液相色谱串联质谱对其浓度进行检测。结果显示,该法中甲醛的检出限为3.06μg/kg,当白菜中甲醛添加含量在1~18 mg/kg时,甲醛线性良好(r2=0.994)。对白菜中3种浓度(0.5、1、5 mg/kg)甲醛添加进行6次平行回收实验,结果表明,3种浓度平行添加回收率在102%~105%,相对标准偏差为3%~8%,回收稳定,精密度良好。In this paper,formaldehyde-2,4-dinitrophenylhydrazone in Chinese cabbage was produced by formaldehyde through 2,4-dinitrophenylhydrazine derivatives methods. Liquid chromatography tandem mass spectrometry analysis results showed that the detection limit of formaldehyde in this method was 3. 06 μg/kg. When the spiking amount in Chinese cabbage was 1-18 mg/Kg,the formaldehyde amount was linearly correlated to peak area（ r2=0. 994）. When the amount of formaldehyde was 0. 5,1,5 mg/kg,recoveries were between 102% to 105%,and the relative standard deviations were between 3% and 8% with good stability and precision.

关 键 词：液相色谱串联质谱 白菜 甲醛 2 4-二硝基苯肼 

分 类 号：O657.63[理学—分析化学] TS255.7[理学—化学]