生物诱抗剂防治大白菜根肿病效果及对根际微生物群落影响  

Control effect of biological resistance inducers on clubroot disease and rhizospheric microbe community of Chinese cabbage

作　　者：刘琴 吴毅歆[3] 张学传[4] 熊菊惠 邓林富 何鹏飞 

机构地区：[1]云南农业大学植物保护学院,昆明650201 [2]云南农业职业技术学院,昆明650213 [3]云南农业大学农学与生物技术学院,昆明650201 [4]云南省禄劝县农技推广总站,禄劝651500 [5]云南省禄劝县云龙乡农业综合服务中心,禄劝651505

出　　处：《华中农业大学学报》2018年第1期32-37,共6页Journal of Huazhong Agricultural University

基　　金：农业部公益性(农业)行业计划项目(201003029);云南省绿色强省计划项目(2009EB060)

摘　　要：为寻找防治大白菜根肿病的更好方法,利用枯草芽孢杆菌XF-1和香菇菌丝体研发生物诱抗剂香菇菌丝体裂解液(EXF-1)和EXF-1丙酮抽提液,并在盆栽条件下测定其防治大白菜根肿病效果及对大白菜根际土微生物群落多样性及土壤微生物量的变化。结果发现:施用EXF-1和EXF-1丙酮抽提物对大白菜根肿病的防效分别达65.07%和42.20%。同时该生物诱抗剂增加了大白菜根际芽孢菌和根际真菌,但减少了放线菌总量,起到调控根际微生态环境、改变土壤微生物群落功能多样性、促进大白菜根生长的作用。Biolog生态板培养表面31个碳源中糖类和氨基酸的方差贡献率分别为35.0%和10.7%。表明施入生物诱抗剂EXF-1和EXF-1丙酮抽提物促进根生长的物质主要是糖类和氨基酸,从而起到调节抑制大白菜根肿病发生的作用。To obtain more measures to controlthe clubroot disease of Chinese cabbage, Bacillus subtilis XF-1 lysis solution of Lentinus edodes mycelia （EXF-1） and its acetone extract were used as bio- logical resistance inducers （BRI） and their effects on controlling clubroot disease and on microbial popu- lation in Chinease cabbage rhizoshere were tested based on the pot culture experiment. The results showed that EXF-1 and its acetone extract could control 65.07%and 42.20% of the clubroot disease, re- spectively. BRIs increased the numbers of Bacillus and fungi, but reduced that of actinomycetes. Based on the results of 120 h culture in Biolog Eco-Plates, sugars and amino acids could explain 35.0% and 10.7% of the variance among 31 principal factors. Therefore, BRI could control the clubroot disease and affect the microbial populations in the soil.

关 键 词：生物诱抗性剂 根际真菌 芽孢菌 生物量 Biolog生态板 

分 类 号：S436.349[农业科学—农业昆虫与害虫防治]