迷迭香多糖的抗疲劳功能及机制研究  

Anti-fatigue mechanism of Rosmarinus Officinalis L Polysaccharides

作　　者：周海洋[1] 

机构地区：[1]漯河医学高等专科学校,河南漯河462002

出　　处：《现代预防医学》2018年第1期43-46,共4页Modern Preventive Medicine

摘　　要：目的观察迷迭香多糖的抗疲劳功能及机制。方法昆明小鼠灌胃不同剂量迷迭香多糖并行负重游泳实验,记录小鼠的游泳时间;行自由游泳实验,检测血清中乳酸、尿素氮、磷酸肌酸激酶、乳酸脱氢酶水平以及腓肠肌中肌糖原含量,比较血清和肝脏中超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-PX)活性和丙二醛(MDA)水平,分析骨骼肌中过氧化物酶体增殖物激活受体γ协同刺激因子1α(PGC-1α)、线粒体转录因子A(mtTFA)蛋白质水平。结果与空白对照组比较,迷迭香多糖能有效延长负重游泳时间,提高自由泳小鼠腓肠肌中肌糖原、血清和肝脏中SOD和GSH-PX的活性,降低血清中乳酸、乳酸脱氢酶、肌酸激酶及血清和肝脏中的MDA的水平,增加骨骼肌中PGC-1α、mtTFA蛋白质水平(P<0.05)。结论迷迭香多糖能通过提供能源物质和减少不利物质以及抗氧化作用消除疲劳,其机制可能与PGC-1α、mtTFA水平改善有关。Objective To observe anti -fatigue mechanism of Rosmarinus officinalis L polysaccharides（RLP）. Methods The mice were sacrificed with different doses of polysaccharide and the mice were subjected to weight - bearing swimming test. The swimming time of mice was recorded. The free - swimming test was performed to detect the levels of lactate, urea Nitrogen, creatine kinase,lactate dehydrogenase in serum and muscle glycogen content in the gastrocnemius were. The levels of superoxide dismutase（ SOD）, glntathione peroxidase （GSH - PX ）and malondialdehyd （MDA）in serum and the liver were compared. The levels of peroxisome proliferator - activated receptor - α coactivator - 1 α （ PGC - 1α）, mitochondrial transcription factor A （mtTFA） protein in skeletal muscle were analyzed. Results RLP could significantly prolong weight - bearing swimming time, increase the content of muscle glycogen in the gastrocnemius, SOD, CAT and GSH of serum and the liver in the mice, decrease the levels of lactate,lactate dehydrogenase, creatine kinase in serum and MDA in serum and the liver, and increase the levels of PGC - 1α, mtTFA protein in skeletal muscle. Conclusion RLP can eliminate fatigue through the provision of energy - substance,reducing adverse substance and antioxidant effect. Its mechanism may be related to PGC - 1α, mtTFA level improvement.

关 键 词：迷迭香 多糖 抗疲劳 抗氧化 PGC-1Α mtTFA 

分 类 号：R15[医药卫生—营养与食品卫生学]